Molecular Surveillance Revealed Increasing Trend of Mycoplasmosis in Respiratory Infections of Goats (Capra hircus) in Northern Pakistan
Molecular Surveillance Revealed Increasing Trend of Mycoplasmosis in Respiratory Infections of Goats (Capra hircus) in Northern Pakistan
Faisal Ahmad1,2, Farhan Anwar Khan1*, Midrar Ullah3, Muhammad Saeed1 and Hayatullah Khan4*
Clinical signs and post mortem examination of naturally infected goats Suspected for CCPP. (A) Mucus plug in nasal cavities, (B) frothy discharges in lower tracheal portion, (C) straw coloured pleural fluid in thoracic cavity, (D) various degree of bilateral hepatisation of lungs.
PPLO broth showing the growth of mycoplasma based on colour change of broth medium (1-4) and 5 negative control.
(A, B, C, D) Growth of mycoplasma colonies (colonies with star) on PPLO agar plate on day 4th-7th after the incubation.
Pure single type of colonies of Mycoplasma mycoides cluster members after 4th passage of the primary culture.
Overall prevalence of the Mycoplasma mycoides cluster and its members in four different zones.
Mycoplasma mycoides cluster recovered from different source of sample. Total number of samples was 2400. Nasal swab n=800, Tracheal swab n=800, Lungs tissue n=400, Pleural fluid n=400.