Screening and Analysis of Immune Genes Related to Disease Resistance in Tibetan Plateau Sheep
Screening and Analysis of Immune Genes Related to Disease Resistance in Tibetan Plateau Sheep
Xiaohui Chen1,2,3, Qing Li1,2, Ming Kang1,2, Xiaolong He1,2, Shuqin Xu1,2 and Guisheng Ye1,2*
ABSTRACT
Total RNA was extracted from healthy spleen tissues from Tibetan sheep of type plateau and small-tailed Han sheep. After mRNA purification and segmentation, cDNA was synthesized for Illumina high-throughput sequencing. The quality of the filtered raw data was evaluated. Subsequently, the cleaned data were used for sequence alignment, KEGG enrichment analysis, differential gene cluster analysis and GO enrichment analysis to screen for disease resistance-related, immune-determinant genes. In addition, the screened differentially expressed genes were verified by Real-Time PCR. The results showed that there were 280 differentially expressed disease resistance-related and immune-related genes in the Tibetan sheep of type plateau compared with those in the small-tailed Han sheep, of which 104 genes were significantly upregulated and 176 genes were significantly downregulated. The results for the GO functional categories showed that the upregulated genes were enriched in the process of immune response, and the downregulated genes were enriched in the extracellular region. The KEGG analysis showed that genes were enriched in 153 KEGG pathways, and the upregulated genes were enriched in pathways involved in antigen processing and presentation and allogeneic rejection. Finally, the real-time PCR results showed that there were 14 differentially expressed genes, with 9 upregulated and 5 downregulated genes. The differential expression trend was consistent with the transcriptome sequencing results.
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