Molecular Studies on Rabies Isolates from Horses in Cairo
Molecular Studies on Rabies Isolates from Horses in Cairo
Yanni, M. I1; Hanaa A. Ahmed2; Lamyaa, A. Ahmed1; Hanan, A. Fahmy3 and Ibrahim, E.M.4 Aggour, M. G.3
ABSTRACT
Rabies causes about 26,000 to 55,000 deaths worldwide per year. More than 95% of these deaths occur in Asia and Africa. In our study, three Arabian horses from Cairo, Egypt, were died after nervous manifestation expecting Rabies. Brain tissues were sent for direct fluorescent antibody technique and isolation on tissue culture using BHK cells. Direct confirmation was performed by Real time RT- PCR and gene sequencing was performed directly from brain samples. Phylogenetic analysis including sequences from all previous Egyptian isolates and neighboring countries isolates was conducted. The % of nucleotide identities of the Egyptian recent isolate (Egy/cairo /2014) with three previous Egyptian isolates were 98.4-98 and 98.4% respectively. Egy/cairo/14 share nucleotide identity with the vaccinal strain flury LEP and kelev with a percentage of (93.1% and 98% respectively). All Egyptian isolates have a common origin. In conclusion ,further epizootological study on a molecular basis is needed.
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