Molecular characterization of IBDV in Egypt, 2015
Molecular characterization of IBDV in Egypt, 2015
Aml S. El-Saadany1, Ayman H. El-Deeb2 and Hussien A. Hussien2
ABSTRACT
Background: Infectious bursal disease virus (IBDV) remerged frequently in Egypt, causing serious
economic losses. In Egypt very virulent (vv) IBDV strains are annually reported since its first
introduction in 1989. Variant IBDV strains were also reported in few studies. To date, IBDV
associated with breaks has been characterized in flocks which were vaccinated with classical IBDV
vaccines and demonstrated IBD characteristic lesions.
Objective: In present study the aim was to molecular characterize IBDV field strains detected in flocks
located in 4 governorates. Also, a phylogenetic analysis based on the sequence of the hyper variable
region of VP2 gene was carried out.
Methods: Forty field bursa samples were collected from different localities. IBDV was detected using
RT-PCR. Sequence analysis of the variable region of VP2 gene purified from the PCR product.
Comparative phylogenetic analysis based on the sequence of the hyper variable region of VP2 gene as
well as phylogenetic tree was constructed along with local and reference IBDV strains using DNAstar
– MegAlign” software.
Results: Eight out of 40 samples were positive for IBDV with RT-PCR, Comparative phylogenetic
analysis based on the sequence of the hyper variable region of VP2 gene showed that the characterized
strains were closely related to IBDV Giza 2008 strains.
Conclusion: this study reports the continuous circulation of vvIBDV strains in vaccinated flocks 4
governorates “Giza, Sharkia, Behira, and Dakahlia”, in Egypt during year 2015. There are need to
review the control strategy and the vaccination failure of IBDV in broiler flocks specially the extensive
abuse of using classical vaccines.
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January 2019
Vol. 5, Pages 1-141
