Occurrence of Borrelia burgdorferi Sensu Lato in Ticks on Camels, along with Risk Factors Analysis in Punjab, Pakistan
Occurrence of Borrelia burgdorferi Sensu Lato in Ticks on Camels, along with Risk Factors Analysis in Punjab, Pakistan
Nadeem Raza1, Aneela Zameer Durrani1, Muhammad Hassan Saleem1, Ali Ahmed Sheikh2, Muhammad Usman1*, Quratulain Mujahid3, Muhammad Zahid Iqbal1, Muhammad Rizwan4 and Muhammad Husnain Ali Alvi1
ABSTRACT
Lyme borreliosis is multisystemic disease caused by Borrelia burgdorferi spirochete, which is tick-borne zoonotic disease of animals and human. It has worldwide circulation including Pakistan. The current study was designed to detect, the B. burgdorferi sensu lato in ticks on camels. Two hundred ticks collected (Bhakkar=100, Bahawalpur=100) from camel in Punjab were examined for potential risk factors, morphological identification, and molecular characterization by using polymerase chain reaction targeting 16S rRNA gene and phylogenetic analysis. Hyalomma dromedarii and Rhipicephalus were found to be 76% (152/200) and 24% (48/200), respectively. Molecular study showed the 10.5% (21/200) prevalence of B. burgdorferi sensu lato in ticks. Phylogeny showed that our isolates branched with isolates from tick (USA) and camel blood (China) with >80% bootstrap consensus. Risk factors examination showed that season, tick infestation and gender are highly significantly (p < 0.05) connected with the presence of B. burgdorferi sensu lato in ticks from camels during field study.
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