Occurrence of Rose Colour Break Tobamovirus in Egypt
Occurrence of Rose Colour Break Tobamovirus in Egypt
Amal Abo El-Ela Ahmed; Eman A.H. Khatab; and M.S. Shafie
ABSTRACT
Rose colour break virus was detected in naturally infected rose growing in Orman Garden for ornamental plants, Giza Governorate and it was found to be widely spread in rose fields. The virus was transmitted mechanically and by grafting (chipbudding) but not by aphids. It was successfully purified from infected N tabacum L. cv. White Burley leaves. Only one band of purified virus preparation was observed 3.5 cm below the meniscus of the density gradient. Infectivity test of the viral zone was found positive. The absorption spectrum of the purified virus isolate had a maximum at 262 nm and a minimum at 251 nm. The max/min and A260/280 ratios of the virus isolate were 1.83 and 2.81 respectively. The yield of purified virus was 6.1 mg/100g or tobacco leaves. Electron micrographs of the purified virus isolate revealed the presence of rod-shaped panicles of 310-320 nm for length and 15 nm for width. The polyclonal antibody raised against the virus under study had a virus specific titer or 1:2000
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