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Optimization Study of Lipolytic Enzyme from Bacillus cereus, PCSIR NL-37

Optimization Study of Lipolytic Enzyme from Bacillus cereus, PCSIR NL-37

Haniya Mazhar1, Naaz Abbas2, Tehseen Zamir3, Zahid Hussain1, Syed Shahid Ali4*

1Institute of Industrial Biotechnology,, GC University, Lahore-54000, Pakistan
2FBRC, PCSIR Labs. Complex, Lahore-54600, Pakistan
3Punjab University Medical Centre, Quaid-e-Azam Campus, Lahore-54590, Pakistan
4Department of Zoology, University of the Punjab, Quaid-e-Azam Campus, Lahore-Pakistan.

*Corresponding Author: Syed Shahid Ali, shahid.zool@pu.edu.pk

 

Figure 1:

Clear zones shown by two lipolytic bacterial isolates on tributyrin-agar plates

Figure 2:

Lipase production by Bacillus cereus PCSIR NL-37 during submerged fermentation

Figure 3:

 Effect of temperature on lipase activity of Bacillus cereus PCSIR NL-37

Figure 4:

Effect of pH on lipase activity shown by Bacillus cereus PCSIR NL-37

Figure 5:

Effect of different inoculum sizes on lipase activity of Bacillus cereus PCSIR NL-37

Figure 6:

 Effect of different carbon sources on lipolytic activity shown by Bacillus cereus PCSIR NL-37

Figure 7:

Effect of nitrogen source on lipase activity shown by Bacillus cereus PCSIR NL-37

Figure 8:

Effect of incubation time on lipolytic enzyme activity shown by Bacillus cereus PCSIR NL-37

Figure 9:

Effect of various metal ions on lipase activity of Bacillus cereus PCSIR NL-37

Figure 10:

Effect of different detergents on lipase activity of Bacillus cereus PCSIR NL-37

Punjab University Journal of Zoology

June

Vol.39, Iss. 1, Pages 01-134

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