Submit or Track your Manuscript LOG-IN

Partial characterization of Necrotic ringspot virus on apple in EGYPT

Partial characterization of Necrotic ringspot virus on apple in EGYPT

Aly M. Abdel-Salam1 and Samah A. Mokbel2

1Plant Pathology Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
2Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center
(ARC), Giza 12619, Egypt.

ABSTRACT

A severe isolate of Necrotic ringspot virus (NRSV) was isolated from apple orchards
in the vicinity of Nubaria city, Beheira governorate, Egypt. Infected-apple trees
showed chlorotic, necrotic ringspots, and shoot holes on leaves. Severely infectedtrees
withered, became useless, and were removed causing severe economic losses.
Reverse transcriptase (RT) polymerase chain reaction (PCR), RT-PCR, using
degenerate primer pair for the coat protein (CP) gene of Ilarvirus amplified products
similar to those produced from peach and apricot isolates of NRSV-infecting stone
fruits. Dot blotting immuno-binding assay (DBIA) showed positive reaction between
NRSV-infected apple sap and an Egyptian antiserum for NRSV. Purified preparation
from infected leaves, using the electro-elution technique yielded nucleoprotein which
had Amax and Amin at 260 and 240 nm respectively. Electron microscopy examination
showed spherical virions with ca. 26 nm in diameter.

To share on other social networks, click on any share button. What are these?

Journal of Virological Sciences

July

Vol. 3, Iss. 1

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe