Pathological and Virological Studies on Bovine Ephemeral Fever
Pathological and Virological Studies on Bovine Ephemeral Fever
Eman, M. Abd El-Mottaleb.*; Ahmed, S.A."; Maysa, H.M.*; Salem, S.A.* and M. M. El-Shenawy
ABSTRACT
The current study submitted in four Egyptian governorates Damietta, Menofia, Fayoum and sharkia. Samples were collected in summer 2006 from dairy cattle showing signs suspected bovine ephemeral fever (BEF). Blood samples on anti-coagulant, nasal swabs and tissues (bovine liver, kidney, lymph nodes and lung) for BEFV isolation and propagation on Vero, BHK21 cell lines and baby mice. Identification was done by both Indirect Fluorescence Antibody Technique (IFAT) on tissue culture and inoculated mouse brain, while immunoperoxidase technique was done on bovine lung. Haematological examination revealed leucocytosis accompanied with neutrophilia (immature form) and lymphopenia. Histopathological examination clarified the most common lesion of field cases in lung in the form of pulmonary emphysema and alveolar collapse as well as interstitial pneumonia and proliferation of the cells lining the bronchioles. Severe edema and infiltration of mononuclear inflammatory cells with proliferative fibrous connective tissues were seen in the heart. Liver showed severe edema, fibrous connective tissues proliferation, various sized organized thrombus with severe infiltration by mononuclear inflammatory cells. Depletion of the white pulp of the spleen as well as edema in the lymph node. The brain of inculcated mice showed intracytoplasmic inclusion body. Electron microscopy (EM) were applied on brain of infected mice and showed intracytoplasmic small number of bullet shape particles.
Virological and pathological results were discussed.
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July 2008
Vol. 5, Iss. 1
