Phylogenetic relationship of some Potyviridae members that found in Egypt.
Phylogenetic relationship of some Potyviridae members that found in Egypt.
Mervat M. Fath- Allah, Amal A. Ahmed, Hala A. Amin
ABSTRACT
Five different plant samples showing different Potyviridae symptoms were previous
isolated and identified. All infected plant samples were examined using ELISA using against, number
of potyviruses polyclonal antibodies. The ELISA results gave a positive reaction with Bean yellow
mosaic virus (BYMV), Carnation vein mottle virus(CVMV), Potato virus Y, Watermelon mosaic virus
(WMV) and Zucchini yellow mosaic virus (ZYMV) of potyviridae family. A potyvirus universal
degenerate primer was used to amplify the polyprotein region of members of Potyviridae. The RTPCR
revealed 335 bp amplified products with only infected plant samples corresponding to viruses
BYMV, CVMV, PVY, WMV and ZYMV, and according to virus symptoms and ELISA results. The
nucleotide sequence analysis of the polyprotein gene of PVY-ME2 (the present Egyptian isolate)
showed 98% homology with the PVY-isolate strain N of Poland. In turn the PVY-ME2 showed low
homology with the other characterized Potyvirus members. Furthermore the PVY-ME2 had much
more distantly related (91%) to the previously published Egyptian and France isolates. The WMVME2
isolate (Egyptian isolate) has a high similarity (98%) with France, Turkey and Chile isolates. On
the other hand, ZYMV –ME2completely separated from MM- isolate of China and it showed only
87% of homology and 98% of identity with JOR-B5 isolate of Jordan. However, the phylogenetic
homology tree based on the multiple sequence alignments revealed that the BYMV-ME2 polyprotein
was closely related to Japanese isolates (S.22C and MB4) since it showed 98 – 97% of similarity . In
turn the BYMV-ME2 appeared far from the BYMV- MC and Alaska isolates of Japan and USA
respectively. Since it showed only 92-89% of identity. The CVMV grouped in PVY group but in a
separate subcluster since it has a slight similarity with PVY virus (66%).
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