Phytochemical Analysis and Biological Evaluation of Methanolic Leaf Extract of Corylus jacquemontii Decne
Phytochemical Analysis and Biological Evaluation of Methanolic Leaf Extract of Corylus jacquemontii Decne
Altaf Ur Rahman1, Muhammad Ajmal Khan2*, Ali Hazrat3, Awais Farid4, Muhammad Yahya3* and Inam Ullah5
ABSTRACT
There is a great concern for healthcare community in antibiotic resistance and evolution of novel bacterial strains. Numerous plants act as a source of novel compounds that could be used to develop new drugs. Therefore, the current study was aimed to investigate the methanolic extract of Corylus jacquemontii for phytochemical, antibacterial, antioxidant potential. The methanolic fraction of C. jacquemontii was evaluated for quantitative phytochemical constituents. The antibacterial efficacy was investigated via well diffusion method by using a concentration of 100 µg/ml. Furthermore, the antioxidant potential of extract was estimated at various concentration (50, 100, 150, 200, 250 µg/ml) through 2, 2-Diphenyl -1- picryl-hydrazyl (DPPH) assay (150 µg/ml). Results showed that the methanolic extract of C. jacquemontii is a rich source of many phytochemically active compounds such as alkaloids content (3.60%), flavonoids (3.40%) and terpenoids 2.30%. The methanolic extract of C. jacquemontii depicted promising antibacterial potential towards various tested bacterial strain in order of P. aeruginosa > E. coli > S. typhi > MRSA comparable to vancomycin. The highest percent mean growth inhibition (MGI) was observed against P. aeruginosa of 90.5±4.3 compared to vancomycin (61.26±6.31). The Zone of Inhibition (ZOI) noted against P. aeruginosa was 20 mm compared to vancomycin (17 mm). The quantitative test antioxidant activity of the methanolic fraction of C. jacquemontii at 250 µg/ml revealed the best antioxidant potential when the DPPH concentration of 150 µg/ml was used. The result showed that extract had a significant effect on antioxidant activity. Therefore, the present study supports the effectiveness of methanolic leaf extract of C. jacquemontii in traditional medicine and may be investigated further for mode of action on molecular basis.
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