Potential Pathological Effects After Repeated Exposure to Tetrodotoxin in Reproductive System of Albino Male Rats
Potential Pathological Effects After Repeated Exposure to Tetrodotoxin in Reproductive System of Albino Male Rats
Taghreed Jabbar Humadai*, Bushra Ibrahim AL-Kaisei
ABSTRACT
The high light of this study was tetrodotoxin has a harmful toxic effect on rats that reflected on oxidative stress and increase IL1 and TNF alpha expression also induce hypospermatogenisis in testes and sperm stasis in epididymis. So, the objective of the present study was to evaluate the toxic effect of tetrodotoxin on the histological structure of male reproductive system and oxidative stress induction in male albino rats. The methods included sixty male albino rats at age 8-9 weeks and weighted 190-200 gm were divided into three groups: The first group: Consisted of 20 rats control were given distilled water and normal rat pellet, the second group: Consisted of 20 rats injected weekly 0.5µg /kg. b.w. by I/P route which represented 1/20 LD50 of tetrodotoxin, the third group: It also consisted of 20 rats injected weekly 1 µg /kg. b.w. by I/P route which represented 1/10 LD50 of tetrodotoxin. At the end of experiment (90 days) all animals were scarified for study the biochemical, histopathological investigations and Immunohistochemistry study. The results of this study showed oxidative stress elevation in Malon Di-Aldehyde concentration in testes, epididymis tissue treated with tetrodotoxin. The pathological lesion in testes of animals exposed to toxic dose of tetrodotoxin was characterized by obvious disruption of seminiferous tubules with absence of leydig cells and congestion, in epididymis tissue the most histopathological section showed widening of intertubular distance with evidence of sperm stasis, focal hyperplasia of epididymal lining with blood vessels congestion and sever mononuclear cells infiltration in the vascular connective tissue, fibrovascular tissue with newly form capillaries between epididymal tubules. The Immunohistochemistry study revealed an increase in the scoring of interleukin1 (IL1) expression in testes and epididymisthat showed the highest score (3), whereas the tumor necrosis factor alpha (TNF α) expression showed highest score (4) in the testes and epididymis tissue. The conclusion of the recent investigation found that tetrodotoxin has a harmful toxic effect on rats that indicated the effects of oxidative stress and increase interleukin1 (IL 1) and tumor necrosis factor alpha (TNF α) expression also induce hypospermatogenisis in testes and sperm stasis in epididymis.
Keywords | Tetrodotoxin, Testes, Epididymis, Pathological changes, Malon Di-Aldehyde, Interleukin 1, Tumor necrosis factor alpha
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April 2024
Vol. 12, Iss. 4, pp. 586-801
