Production of FMDV ELISA Kit for Antibodies Detection in Animals Using Native Strain with Production of Reference Hyper-immune Serum in Guinea Pigs
Production of FMDV ELISA Kit for Antibodies Detection in Animals Using Native Strain with Production of Reference Hyper-immune Serum in Guinea Pigs
M. E. Shawky and A. M. Daoud
ABSTRACT
The objective of the present study was to evaluate an enzyme linked immunosorbent assay (ELISA) based kit for detecting foot and mouth disease (FMD) antibodies using a native FMDV strain (O1. Aga 93). Results were compared to those of serum neutralization test (SNT) and 3CD ELISA Kit. Sensitivities of the ELISA Kit and 3CD ELISA were 94% and 91%, respectively. Rapid confirmation of the presence of FMDV antibodies through the developed ELISA Kit may have a significant impact on initiating emergency control of FMD outbreaks.
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December 2004
2004
