Protagonist Action of Plant Growth Regulators and Sublethal-Temperature in Inducing Thermotolerance in Green Gram (Vigna radiata) Seedlings
Protagonist Action of Plant Growth Regulators and Sublethal-Temperature in Inducing Thermotolerance in Green Gram (Vigna radiata) Seedlings
Simeen Mansoor, Jabeen Farheen* and Meher Hassan
ABSTRACT
The specific proteins induced by sublethal-temperature are molecular chaperons that positively regulate plant growth and development that govern acclimation in plants but little has been known under lethal-temperature stress. Thus, the impact of induction of thermotolerance by sublethal-temperature (40 °C), 100 µM indoleacetic acid (IAA), and 100 µM gibberellic acid (GAᴣ) before lethal-temperature stress (50 °C) were assessed on green gram growth and protein profile. For this purpose, the distilled-water and phytohormones imbibed seeds of NM13-1 were pretreated with 40 °C (1 hour) before 50 °C (2 hours), after 24 hours seedling length, proteome profile by 1-D, and catalase (CAT), ascorbate (APX), and guaiacol peroxidase (GPX) activities were assessed. Study outcomes revealed that the combination of phytohormones and 40 °C before 50 °C exhibited improved growth and increased activity of APX, CAT, and GPX as to control and lethal-temperature treatments. The total soluble protein profile showed highly significant variations and was broadly divided into two major clusters. The 116, 113, 109, 106, 94, 86, 76, 62, and 61 kDa fractions were identified the first time, which may be involved in temperature stress acclimation. Conclusively, the sublethal-temperature along with phytohormones are involved in green gram growth, proteome expression, and thermotolerance via initiating antioxidant defense signaling.
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June 2021
Vol. 7, Iss. 1, Pages 1-205
