SYMPTOMATOLOGY AND SEROLOGICALL SURVEY OF VIRUSES INFECTING SUGAR BEET CULTIVATION IN EGYPT
SYMPTOMATOLOGY AND SEROLOGICALL SURVEY OF VIRUSES INFECTING SUGAR BEET CULTIVATION IN EGYPT
Megahed, A.A.1; Kh. A.El-Dougdoug2 and B.A. Othman2
ABSTRACT
A survey was conducted in the main Egyptian sugar beet growing areas to determine the occurrence and incidence of Cucumber mosaic cucumovirus (CMV), Beet mosaic potyvirus (BtMV), Beet necrotic yellow vein benyvirus (BNYVV), Beet curly top geminivirus (BCTV) and Beet yellows closterovirus (BYV) symptomatology and serologically by double antibody sandwaich Enzyme linked immunosorbent assay (DAS-ELISA). The survey of sugar beet viruses achieved in 15 field locations belonging to 5 different Governorates, which performed by examination 915 plant samples from 149 fields in two seasons (2009/2010 and 2010/2011) symptomatology. During survey, 464 of naturally infected sugar beet plants showing distincted for one or more viruses were collected from 73 fields in season 2009/2010, while from 76 field, the total of examined plants were 451 through the second season (2010/2011), upon external symptoms. All samples were distributed in 8 symptomatic groups in the forms of: vein clearing, mottling, mosaic, blisters, leaf curling, stunting, yellowing and necrosis. Depending on the external symptoms, the present study revealed that, the total of expected and recorded viruses was five sugar beet viruses (CMV, BtMV, BNYVV, BCTV and BYV). The highly incidence percentage was recorded to BCTV (53.3 and 47.2 %) for both seasons (2009/2010 and 2010/2011). The values of ELISA conformation of total tested 240 of 915 samples of naturally cultivated plants were detected serologically by DAS-ELISA for CMV, BtMV and BNYVV infection were 225 samples reacted positively. The total positive samples were 112 samples for season 2009/2010 and 113 positive ones for season 2010/2011. The total positives for individual CMV infection were 45 and their highest frequency percentage was 29.79 % of the samples collected from El-Gharbia Governorate. The total positive individual BtMV and BNYVV infections were 59 (35.71 %) and 13 (11.90 %) respectively collected from El-Behiera Governorate.
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