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Screening of Puberty Related Differentially Expressed Genes from Ovary Tissues of Jining Grey Goat Based on Suppression Subtractive Hybridization

Screening of Puberty Related Differentially Expressed Genes from Ovary Tissues of Jining Grey Goat Based on Suppression Subtractive Hybridization

Yufang Liu1,2, Guiling Cao3, Yujing Xie3 and Mingxing Chu1*

1Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
2College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056021, China.
3College of Agriculture, Liaocheng University, Liaocheng 252059, China.
 
Yufang Liu and Guiling Cao made equal contributors to this article.
 
*      Corresponding author: mxchu@263.net
0030-9923/2023/0004-1637 $ 9.00/0

Fig. 1.

Agarose electrophoresis analysis of optimal cycles of double-stranded cDNA. Electrophoresis with agarose of 1.2% concentration. M: DNA marker DL 2000 (100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 2000 bp).

Fig. 2.

GO classification of differential expression genes from the three groups of ovaries. AO, BO and EO represent the three groups in Table IV, respectively.

Fig. 3.

KOG classification of differential expression genes from the three groups of ovaries. AO, BO and EO represent the three groups in Table II, respectively.

Fig. 4.
KEGG pathway enrichment analysis of SSH differential genes. AO, BO and EO represent the three groups in Table II, respectively.
Fig. 5.

The protein-protein interactions of differential expression genes from the three group of ovaries. Genes in the first red circle participate in ribosome/protein translations, genes in the second red circle participate in oxidative phosphorylation and genes in the third red circle participate in carbohydrate metabolism.

Fig. 6.

The relationship prediction between CYP11A1 and prolificacy-associated markers among four species. A: cattle; B: human; C: mouse; D: goat.

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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