The Influence of Gibberellic Acid (GA) and Indole Acetic Acid (IAA) on The Production of Local Lesions on Nicotiana sylvestris Plants Infected with Tomato Mosaic Tobamovirus (ToMV)
The Influence of Gibberellic Acid (GA) and Indole Acetic Acid (IAA) on The Production of Local Lesions on Nicotiana sylvestris Plants Infected with Tomato Mosaic Tobamovirus (ToMV)
E.F. Mohamed1 and I.M. Sabra2
ABSTRACT
Tomato mosaic disease caused by Tomato mosaic (ToMV) is widespread wherever tomato is grown ToMV affects plants and yields. Virus was isolated and identified on the base of symptomatology on tomato plants, host range, diagnostic hosts, physical ptoperties, mode of transmission, and ELISA detection. ToMV had a longevity in vitro (LIV) of 90 days at room temperature. dilution end point (DEP) Of 10-6 and thermal inactivation point (TIP) of 90oC. ToMV was easily transmitted by sap. The obtained results revealed that ToMV was not transmitted by any insects used in this study. The identification of ToMV was confirmed serologically using ELISA technique. The effectiveness of gibberellic acid (GA) and indole acetic acid (IAA) against Tomato mosaic virus (ToMV) in vitro and in vivo has been evaluated. GA and IAA reduced in vitro and in vivo the infectivity of ToMV to a certain extent expressed as the number or local lesions induced by ToMV on Nicotiana sylvestris. This reduction increased gradually by increasing GA and IAA concentrations. ToMV inhibition percentages induced by GA and IAA varied according to the time of treatment (1, 2, 3, and 4 hours). High percentages of inhibition were recorded for in Vitro treatment. ToMV inhibition of pre-inoculation treatment was higher than that of post-inoculation treatment. IAA was more effective than GA.
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