The Transcriptional Regulation Mechanism of Transgelin Gene in Colon Cancer Cells
The Transcriptional Regulation Mechanism of Transgelin Gene in Colon Cancer Cells
Qi Zhuo, Yuanchun Yao, Meisongzhu Yang* and Jinhua Chen and Miao Tian
ABSTRACT
The objective of this study was to explore the transcriptional regulation mechanism of transgelin gene in colon cancer cells. HT29 cells were randomly divided into three groups: CON group, TGT group and DGT group. The transgelin gene was sub-cultured in the control (CON) group, transfected in the TGT (cells transfected with tranglin gene) group, and knocked out in the DGT (cells in which tranglin gene has been knowcked out) group. The protein expression and the gene expression were measured using Western blotting and RT-QPCR, respectively. We found that cell proliferation rates in CON, TGT and DGT groups showed that cells were transfected with trangelin gene, the cell proliferation rate died down, significantly lower than that in CON and DGT groups (p<0.05). Cells migration and invasion results in all groups showed that after cells were transfected with trangelin gene, the number of migrated cells decreased significantly lower than in CON and DGT groups (p<0.05). Cells invasion results showed that after cells were transfected with tranglin gene, the number of invasion cells decreased significantly lower than that in CON and DGT groups (p<0.05). RT-PCR results showed that the transgelin gene in both CON and TGT groups was significantly increased, much higher than that in the CON group. The EST-1 gene expression in the TGT group was significantly reduced, lower than that in the CON group. WB results showed that the transgelin gene in ERK, AKT, MMP and VEGF proteins in CON and TGT groups was subjected to low expression, whereas in the DGT group it was higher than that in the CON group (P<0.05). It is concluded that transgelin gene can block the transcription of HT29 cells in colon cancer, thus affecting the normal proliferation and differentiation of cancer cells.
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