To develop the effective methods for the innocuous treatment of the poultry manure, this study explored the efficiency of bacteria in decomposing the poultry manure through testing the variation of gas production and nitrogen(N), phosphorus (P), potassium(K)in the fermentation process. Poultry litter, sawdust and rice husk were mixed in equal quantities by volume. One was treated with a single strain of Bacillus; another was treated with a consortium of bacteria comprising several species; and the third kept as the control without adding any bacteria. The results showed that decomposition started earlier in the treated batches than in the control group. During the fermentation period, there was no influence between the internal temperature of the plies and the addition of bacteria. After adding strains, the time to reach the fermentation temperature was advanced by 1 to 2 days. The content of ammonia and hydrogen sulfide were increased at the first weekend, reaching up to 10ppm and 3.5 ppm respectively. Three weeks later, the content of the ammonia (NH3) and hydrogen sulfide (H2S) were decreased to 0.3 ppm and 0.6 ppm respectively. Total N, P and K were increased gradually with time, whereas the content of ammonium nitrogen was decreased. SAS software analysis showed that there was no significant difference in H2S, CO2 and NH3 between the three groups (p>0.05), the same results showed that N, P, K, organic matter and ammonium nitrogen had no significant difference(p>0.05). These results suggested that the inoculation with Bacillus genus bacteria improved the microbial proliferation and shortened the time that the substrate reached to the fermentation temperature, resulting in the retention of N, P, K and the reduction of the NH3, H2S from the fermentation litter.