Trials for preparation of inactivated avian influenza vaccine from local isolate H5N1
Trials for preparation of inactivated avian influenza vaccine from local isolate H5N1
Walaa Abd El-Fatah S. Metwally1, Ismail M. Reda2, Ahmed A. El-Sanousi2, Mohamed Abd El-Khalek Ali2
ABSTRACT
In the present study avian influenza (AI) inactivated vaccine was prepared with Montanide ISA70VG which has been added as adjuvant using reassortant AI strain H5N1. The prepared vaccines with Montanide ISA70VG varied in the HA units per dose and also differed in the ratios between two reassortant strains (Chicken and duck strains) of AI. The results of testing humoral immunity revealed that the prepared vaccines with Montanide ISA70VG containing 350 HA unit/dose were high in antibody titer than vaccines containing 256 and 100 HA unit/dose. The prepared vaccines with Montanide ISA 70VG containing 256 HA unit/dose gave high antibody titer than vaccines containing 100 HA unit/dose. The ratio of 30% Chicken strain of AI to 70 % Duck strain of AI gave the best result of antibody titer followed by the ratio 40% to 60% followed by 50% to 50% respectively. The highest titer observed in the prepared vaccine was in the ratio of 30% Chicken strain of AI to 70% Duck strain of AI containing 350 HA unit/dose after 3 weeks post vaccination and remained high till 30 weeks post vaccination with protective antibody titer and protection against challenge followed by the ratio of 30% Chicken strain to 70 %Duck strains of AI containing 256 HA unit/dose which gave high antibody titer after 4 weeks post vaccination and remained high till 26 weeks post vaccination followed by the ratio of 30% Chicken strain to70 %Duck strain of AI containing 100 HA unit/dose which gave high antibody titer 5 weeks post vaccination and remained high till 20 weeks post vaccination. The results of testing cell mediated immunity revealed that the prepared vaccines containing 350 HAU/dose induced significant high lymphocyte cells followed by 256 HAU/dose followed by 100 HAU/dose. The challenge test revealed that the prepared vaccine in the ratio of 30% Chicken strain of AI plus 70% Duck strain of AI containing 350, 256 and 100 HA unit/dose showed protection reached to 85%, 80% and 30% respectively. In conclusion, the study highlight the add value of using different HA units/dose of AI and different ratios of the two strains (chicken and duck strains).
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