Utilization of SSR Markers to Identify Slow Rusting Genes in Spring Wheat (Triticum aestivum L.)
Utilization of SSR Markers to Identify Slow Rusting Genes in Spring Wheat (Triticum aestivum L.)
Ghassan Zahid1, Sania Begum2, Sikandar Almani3, Sahir Hameed Khattak2, Rajesh Kumar Soothar4 and Shakeel Ahmed Soomro4*
ABSTRACT
The virulence of rust diseases in wheat crop like leaf, stripe and stem-rust declines the grain quality and productivity. Cultivars having a durable rust resistance is an effective method to control the spread of rust disease in cereal crops particularly in wheat. The experiment was conducted on fifty-two wheat lines comprised of forty-five double haploids of wheat along with seven wheat genotypes. Besides that, three positive checks and three SSR primers were also included in the study to check the durability i.e. Opata-85 Sr2/Lr27check, Pavon-76 (Yr-29/Lr-46) and Tukuru (Yr18/Lr34) respectively. The molecular markers screening results indicated that the primer Sr2/Lr27 was present in almost all of the double haploids and genotypes except the Nesser and Tukuru varieties. The Yr29/Lr46 gene complex was present in all double haploids and genotypes except for Nesser, Opata-85, inqilab-91 and Tukuru. the gene complex Yr18/Lr34 was present in eight double haploids, and only one genotype showed its presence, however the rest of the genotypes lacked this gene complex. The obtained results intimated that the 8 double haploids 33, 34, 44, 45, 46, 48, 53, 54, and genotype weebil-1 contained all the slow rusting genes complex i.e. Yr18/Lr34, Yr29/Lr46 and Sr2/Lr27. Therefore, detection of slow rusting gene complex in these varieties with the assistance of molecular markers can be utilized for slow-rust resistance. Furthermore, the selected markers can be used as a primary choice for detecting rust resistance genes specifically in the wheat breeding population.
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March 2022
Vol. 35, Iss. 1, Pages 1-258
