Jiancheng Zhai, Li Gao, Yanling Xia and Heping Li*
... which the females carry antlers in Cervidae, and the differences between the antler tips of the two sexes are one of the most interesting scientific mysteries. The objective of this study was to isolate preliminarily differentially expressed genes in antler mesenchyme of both sexes. The genes were detected with three anchored primers and ten random primers using differential display rever...
Yanling Xia1,2, Heping Li2, Yuntao Liang2, Jichen Zhao2, Binshan Lu2 and Di Liu1,*
...successfully cloned from antler tip of the Sika deer (Cervus nippon hortulorum). The Vps26A cDNA contains an open reading frame of 984bp encoding a polypeptide with 327 amino acids. The deduced molecular mass and isoelectric point of Vps26A protein were 38.2 kDa and 6.13, respectively. Glutamic acid had the largest proportion (10.4%) in the primary structure. Homologous sequence alignment and phylogenetic tree analysis indicated that theVps26A protein o...
Nina Bao1,*, Yongguang Yin2 and Panpan Wang3
...tituent of cervus nippon antler velvet layer was studied in this paper. Single-factor experiment was used to research the effect of liquid-to-solid ratio, standing time and centrifugation time on the extraction of crude cerebroside. The optimal extraction conditions for the cerebroside as follows: liquid-to-solid ratio 15, standing time 45 min, and centrifugation time 6 min. The extracted cerebroside was purified and identified by using silica gel column and T...
Lin Qi, Lu Li, Danyang Chen, Mingxiao Liu, Yan Wu and Wei Hu*
...l monomeric peptide from antler plate polypeptide (APP-1) of sika deer which inhibited the growth of both Gram-positive and Gram-negative bacteria. The molecular mass and purity of this polypeptide was determined by ultra-performance liquid chromatography (UPLC) and Bruker micOTOF OllQ TOF mass spectrometry, respectively. The full amino-acid sequence of the monomericpeptide was analyzed by sequential Edman degradation using a protein/peptide sequencer. The ant...
Yan Xu1, Jia Zhou1, Guangfu Lv2, Yuexin Liu1, Xintong Zhao1, Xin Li1, Doudan Ye2, Xiaobo Qu1,* and Xiaowei Huang1*
...es the effects of pilose antler peptide (PAP) on DOX-induced myocardial injury and related mechanisms through in vitro experiments. Different concentrations of DOX were used to treat H9c2 cells for 6, 12, 24, and 48 h, respectively, to determine the IC50. Different concentrations of PAP were treated to H9c2 cells for 24, 48, and 72 h to determine the effect of PAP on H9c2 cells. The experiment was divided into control group, DOX group, PAP group and...
Jian-Cheng Zhai1,2, Sheng-Nan Wang1,3, Qiang-Hui Wang1, Yan-Ling Xia1, Wei-Shi Liu1, Ya-Jie Yin4 and He-Ping Li1*
...ecies in both sexes grow antlers. Many apparent differences exist in antlers of female and male reindeer and sika deer. Insulin like growth factor 1 (IGF1), Keratinocyte growth factor (KGF) and Nerve Growth Factor (NGF) are essential for antler growth. To investigate whether epigenetic regulation of the growth factors is important in growth of sika deer, female and male reindeer
Muhammad Nauman Arif1, Muhammad Mansha1* and Tanveer Hussain2
...ue to its meat, skin and antlers, but there are insufficient molecular data, provoking us to explore its genetic variation and phylogenetic analysis using ATPase 6/8 genes of mitochondrial DNA. The blood samples from hog deer were collected and DNA was extracted by using in-house organic methods. PCR was used to amplify a total of 880 bp of overlapping genes, which were then sequenced. Bioedit software was used to align, and edit the sequences, and single nucl...
Ying Chen1, Chao-Zheng Li2, Zhun Yu3, Jia Zhou4, Yan Xu4, Zhe Lin4, He Lin4* and Xiao-Wei Huang4*
...-align: justify;">Velvet antler polypeptide (VAP) is the active compounds of velvet antler, an animal derived traditional Chinese medicine. In this work, the myocardial ischemia-reperfusion injury (MIRI) model was established to investigate the protective mechanism of VAP on MIRI in rats. Total 90 male Sprague Dawley (SD) rats were divided into the control, model (MIRI), positive (Diltiazem, DLZ, 20 mg/kg), VAP high, medium,...
Xiao-Wei Huang1,2, Mei-Li Liu1, Jin-Ji Wang1, Yue-Xin Liu3, Zhe Lin1, Chun-Shu Rong4* and Ji-Xiang Ren4*
... the mechanism of velvet antler polypeptide (VAP) in improvement of learning and memory function in mild cognitive impairment (MCI) rats model, specifically by investigating the PI3K, AKT and mTOR pathway. The type and content of amino acids (AA) in VAP were determined using pre-column derivatization-high performance liquid chromatography (PD-HPLC). Wistar rats were randomly divided into 5 groups (n=10 per group): control, model, piracetam (PIR), VAPH (300 mg/...
Hong Chen1,2, Jiawei Liu1,2, Chuan Lin1,2, Hao Lv1,2, Jiyun Zhang1,2, Xiaodong Jia1,2, Qinghua Gao1,2 and Chunmei Han1,2*
...ique mammalian appendage antler is a good model for studying tissue regeneration and its related mechanism. This study explored the mechanism of the rapid growth of regenerated antlers after top pruning damage using transcriptome sequencing technology. A damage repair model was constructed by top pruning treatment on the left antlers of three 10-month-old Tarim red deer, and RNA-seq analys...
Pakistan Journal of Zoology
November
Featuring
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Morphometric Measurements, Gonopod and Gonopores Appearances in Early Sexual Crablet Stage of Scylla paramamosain (Estampador, 1949)
Muhammad Nur Syafaat, Che Zulkifli Che-Ismail, Adnan Amin-Safwan, Mohamad N. Azra, Mohammad Syahnon, Ambok Bolong Abol-Munafi and Mhd Ikhwanuddin
Pakistan J. Zool., Vol. 57, Iss. 2, pp. 759-766
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Preparation of High Selenized Gastrodia elata Blume Polysaccharide and its Immunomodulatory Effects on RAW264.7 Cells and Cyclophosphamide-Treated Mice
Fengwei Ma, Qihua Wen, Qingfang Deng, Yihao Lu, Buyan Zhang, Yongyou Cheng and Su Xu
Pakistan J. Zool., Vol. 57, Iss. 2, pp. 747-758
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Freshwater Fish Biodiversity in Liangzi Lake
Ke Li, Hai Liu, Jiangang Zhou, Hongbing Chen and Zhengxiang Wang
Pakistan J. Zool., Vol. 57, Iss. 2, pp. 737-745
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Histopathological and Molecular Analyses of Leptospira borgpetersenii and Leptospira interrogans in Bovine Kidneys in Kota Bharu, Kelantan, Malaysia
Intan Noor Aina Kamaruzaman, Siew Zee Ong, Iman Natasha Sofea Jafri, Dayangku Umi Aqilah Pengiran-Isa, Mohamad Sabri Abdul-Rahman, Mohd Farhan Hanif Reduan, Thilini Nisansala, Soon Heng Goh, Siti Nur Haslina Mamat, C.W. Salma C.W. Zalati, Sazaly AbuBakar and Shih Keng Loong
Pakistan J. Zool., Vol. 57, Iss. 2, pp. 727-736
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