Histopathological and Molecular Analyses of Leptospira borgpetersenii and Leptospira interrogans in Bovine Kidneys in Kota Bharu, Kelantan, Malaysia
Histopathological and Molecular Analyses of Leptospira borgpetersenii and Leptospira interrogans in Bovine Kidneys in Kota Bharu, Kelantan, Malaysia
Intan Noor Aina Kamaruzaman1*, Siew Zee Ong2, Iman Natasha Sofea Jafri2, Dayangku Umi Aqilah Pengiran-Isa2, Mohamad Sabri Abdul-Rahman3, Mohd Farhan Hanif Reduan2, Thilini Nisansala1, Soon Heng Goh1, Siti Nur Haslina Mamat2, C.W. Salma C.W. Zalati3, Sazaly AbuBakar4 and Shih Keng Loong4*
ABSTRACT
Leptospirosis is a zoonotic bacterial disease affecting both humans and animals. This is the first study aiming to detect the Leptospira spp. in bovine kidneys collected from selected wet markets in Kota Bharu, Kelantan and subsequently determine the predominant Leptospira species. Additionally, the pathological alterations to the bovine kidneys as a result of leptospirosis were also analyzed. A total of fifty bovine kidney samples (n=50) were collected from four wet markets within Kota Bharu town and the samples were tested using a polymerase chain reaction (PCR) assay. From the results, thirteen samples (n=13; 26%) were found to be positive for the 16S rRNA gene. DNA sequencing of the positive samples revealed the presence of Leptospira borgpetersenii (n=11) and Leptospira interrogans (n=1), which are known to be pathogenic Leptospira species in cattle and humans. One sample cannot be sequenced due to poor yield and quality. Furthermore, the kidneys that were PCR-positive showed significant histopathological lesions of bovine leptospirosis, consisting of interstitial nephritis, glomerular atrophy and tubular necrosis. In conclusion, the present study demonstrated the presence of L. borgpetersenii and L. interrogans in local cattle and this is an indisputable public health risk to the public considering that the samples were obtained from the local wet markets.
To share on other social networks, click on any share button. What are these?
