Search for Capsid

Antigenic Domains of Avian HEV: Bases for Serological Diagnosis of HEV in Avian, Swine and Human

Qin Zhao^1,2, Yani Sun^1,2, Gaiping Zhang^1,3, Frederik Widen⁴, En-Min Zhou^1,2,*

[email protected]

... of HEV.

...

A Virus-like Particle Vaccine Against Infectious Bursal Disease Virus: Potential Uses and Applications

Kang-Seuk Choi

...s) comprising only viral capsid proteins mimic the naive configuration of authentic IBD virus particles. The VLPs show intrinsic immunogenicity and a high safety profile. Thus, VLPs are considered one of the most promising approaches to vaccine development and are an alternative to inactivated IBD vaccines. In addition, VLP technology has many applications. This paper reviews the potential of VLPs as an alternative to IBD vaccines, along with their speci...

Human Cytomegalovirus Tegument Protein pUL23 Interacts with Capsid Protein pUL85

Shaoling Lin and Jiamiao Hu

...n essential component of capsid, the minor capsid protein (mCP, pUL85). Interaction was determined and confirmed with yeast two-hybrid, GST pull-down and co-immunoprecipitation analyses. This interaction may serve as a link in the processes of HCMV capsidation and tegumentation. This study provides further evidences in the functions of tegument proteins in the assembly of HCMV virons.

...

Canine Coronavirus (CCoV), a Neglected Pathogen: Molecular Diversity of S, M, N and 3b Genes

Iracema Nunes de Barros^1,2, Luciane Dubina Pinto³, Rosely Bianca dos Santos Kuroda¹, Sheila Oliveira de Souza Silva¹, Leonardo José Richtzenhain^1,2, Cláudio Wageck Canal³ and Paulo Eduardo Brandão^1,2*

...ne (M), spike (S), nucleocapsid (N) and non-structural protein 3b genes. Out of the samples collected, 40.17% were positive for CCoV; 57.45% of CCoV-infected animals showed enteritis and most of these (76%) were younger than 3 months and unvaccinated. Distance genealogy using CCoV sequences from GenBank for M gene showed that eight strains were CCoV-II twenty-six were CCoV-I. These findings show some genetic features of CCoV in Brazil and may require future st...

Molecular Detection of Hepatitis E Virus in Layer Chickens: A Possible Public Health Risk in Pakistan

Tahir Iqbal¹, Umer Rashid¹*, Naveed Shahzad², Amber Afroz¹, Muhammad Faheem Malik³ and Muhammad Idrees⁴

...6 bps, 2769-2954 nt) and capsid (280 bps, 5461-5741 nt) domain in the bile fluids of two birds. The histological data demonstrated pathological changes in liver and spleen tissues of layer chickens positive for viral RNA. The extensive phylogenetic analysis on the basis of partial helicase domain (ORF1) and capsid protein (ORF2) revealed clustering of Pakistani aHEV (Pak aHEV) strains with members of Orthohepevirus

Isolation and Complete Capsid Sequence of Enterovirus D111 from Faeces of a Child with Acute Flaccid Paralysis in Nigeria

Faleye Temitope Oluwasegun Cephas^1,2*, Adewumi Moses Olubusuyi¹, Olayinka Olaitan Titilola³ and Adeniji Johnson Adekunle^1,3

...n and the first complete capsid sequence of the virus globally. We also show that NR-CPE could sometimes be caused by EVs that do not produce R-CPE in specific cell lines.

...

Characterization of Unusual Human Group A Rotavirus VP4 Genotype Detected in Moroccan Children Fully Vaccinated with Rotarix™

Hassan Boulahyaoui^1,2*, Sanaa Alaoui Amine^1,3, Marouane Melloul⁴, Farida Hilali⁵, Elmostafa El-Fahime^1,3, Saad Mrani^1,2 and Nadia Touil^1,5*

...nic regions of the outer capsid proteins VP8* were compared with vaccine and field strains. Here we show that the VP4 gene of the P[14] strains detected in this study exhibited close identity with zoonotic Moroccan goat and bovine strains. The amino acid sequences of the antigenic regions inside VP8* proteins showed high conservation between the amino acid sequences of human and animal strains bearing P[14] proteins, and revealed several changes with respect t...

Genomic Analysis of Nucleocapsid and Fusion Genes of Small Ruminant Morbillivirus in Pakistan

Hafiz Muhammad Imran Javed* and Mushtaq Ahmed

Isolation and Molecular Characterization of Bovine Coronavirus from Calves with Acute Gastroenteritis in Egypt

Saad A. Moussa¹, Ahmed F. Afify¹*, Suzan Salah² and Ayman Hamed³

...RT-PCR targeted to nucleocapsid protein gene (N-gene) was carried out followed by phylogenetic analysis. Positive samples were isolated on Vero cell culture and identified by TEM and immune-peroxidase technique. Betacoronavirus 1 was detected by ELISA in 6 out of 20 fecal samples (30%), PCR detected 4 out of 6 ELISA positive samples at specific M.W. band of 236 bp by electrophoresis, and one sample was sequenced and submitted on Genbank with acc.no. MW173144, ...

ISOLATION OF AN ENTOMOPATHOGENIC VIRUS IN THE GREEN STINK BUG NEZARA VIRIDULA (LINNAEUS) IN EGYPT

AdlyAbd-Alla1,2, MahaNada3, Francois Cousserans1, and Max Bergoin1

...r genome and analysis of capsids showed two major proteins of 31 and 32 kDa

and a minor 78 kDa protein. Infection of third instar nymphs by feeding on contaminated food

induced similar disease symptoms and bioassay experiment confirmed virus concentration

response mortality. Injection of a purified virus suspension to third instar larvae of Galleria

melonella and Spodopteralittoralis failed to induce any mortalit...

A genotyping of a new avian infectious bronchitis virus isolated from chickens proventriculus in Egypt

Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1

...-PCR targeting the nucleocapsid (N) gene with further genetic and antigenic characterization. Methods: Beside a real time RT-PCR; sequence analysis of partial S1 gene, cross-neutralization and the challenge experiment for H120 vaccinated broiler chickens were also performed. Results: Egypt/Qal/014p had close relation with that of the Massachusetts prototypes. Comparisons nucleotide and amino acid sequence of partial S1 gene showed that the recent isolate had 9...

A Theory of Recombinant Virus Particle Formation

Daniel Stewart Robertson

... to be hydrolysis of the capsid by monophosphoric acid. Also shown is that for protection against the common cold an individual has ideally to be vaccinated with attenuated versions of the virus particles produced by his or her own cells. Observations supporting the proposals are presented.

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Wildlife as a Source of SARS-CoV-2 Evolution- A Review

Muhammad Altaf¹* Arshad Mahmood Abbasi², Muhammad Shoaib Amjad³, Sadia Naseer¹ and Muhammad Umair⁴

.... envelope, spike, nucleocapsid and membrane. Coronaviruses are classified into 4 genera: Alphacoronavirus, Betacoronavirus, Gammacoronavirus and Deltacoronavirus. Betacoronavirus most probably originated from bats and the virus may have jumped to avian species and evolved as Deltacoronavirus group. The avian coronaviruses jumped among other avian species, giving rise to Gammacoronavirus from Deltacoronavirus, while Betacoronavirus may have given rise to Alpha...

Molecular Cloning and Expression of the Coat Protein Gene of Plum Pox virus El-Amar Strain in E. coli

M. A. Abou El- Nasr1, Kh. A. Dougdoug1, Hayam S. Abdelkader2, and Rehab A.

Dawoud2

...-terminal portion of the capsid protein of PPV. The amplified products were cloned into pGEM-T-Easy vector and hybridized to PPV DNA specific probe labeled with Dig-I 1dUTP. DNA sequencing using fluorescent dideoxy nucleotides showed that the capsid protein region of PPV-EA strain had about 65% sequence homology with other strains of PPV and 45% similarity to the CP or PPV-D strain. A PCR fragment coding for the 43 C-termina...

Detection of Grapevine Fanleaf Virus Capsid Protein Gene by RT-PCR and DNA hybridization

E. K. Allam.1; B. A. Othman.1; Hayam S. Abdelkader2; and Noher A. Mahmoud2

Characterization and Molecular Detection of Tomato spotted wilt Tospovirus Infecting Tomato in Egypt

Hayam S. Abdelkader1; Gamalat M. Allam1; T. A. Moustafa2; and M. El-Hanunady2

...mplementary to the nucleocapsid protein gene (NPs) were used to detect two isolates of TSWV from Lycopersicum esculentum and Physalis peruviana plants. Total nucleic acids were reverse transcribed using Retrotool reverse transcriptase enzyme and the PCR reactions were performed for 30 min in a capillary thermal cycler. RFLP analysis of the PCR products was performed using Msel restriction enzyme. The results showed a dimorphic restriction digestion profi...

Preliminary Exploration of Transcriptome Comparative Analysis of Muscle Tissues from Asymptomatic and Diseased Epinephelus fuscoguttatus Infected with Nervous Necrosis Virus

Wei Fang^1,2,3, Jiawei Hong^1,2,3,4, Zhengyi Fu^1,2,3, Jing Hu^1,2,3, Gang Yu^1,2,3, Zhenhua Ma^1,2,3* and Humin Zong⁵*

...to the inhibition of the capsid protein encoded by NNV’s RNA2. This research not only provided a theoretical basis for the breeding of anti-NVV, but also made up for the gap in the research field of anti-NVV of E. fuscoguttatus.

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Engineered Norovirus-like Particle as a Vaccine Candidate

Shawki, Khaled K.; Carter, M.J.; Alnashar, Nariman M., El-Farrashl, Mohamed A. and Taher, Sahar

...lf-assembly of the virus capsid protein. In this study we employed a new approach for engineering stable particles of Hawaii virus capsid protein by deleting those immunodominant regions (P2subdomains) that evoke type-specific responses and bridging the resulting gap with a synthetic poly-glycine chain. This construct was expressed using the baculovirus system and the obtained purified protein was examined by EM which showed...

Baculovirus Expression of the Bovine Coronavirus Nucleocapsid Protein in Spodopetra Frugiperda Insect Cells

Amer, H.M.; Hussein, H. A.; El-Sabagh, I. M.; El-Sanousi, A. A. Saber,M.S. and Shalaby, M. A.

... coronavirus (BCV) nucleocapsid protein was carried out in a baculovirus expression system. The specific RT-PCR product of N gene was cut and extracted from gel using DNA gel extraction kit (Millipore). Eluted DNA was successfully cloned in pBlueBac4.5N5-His TOPO TA baculovirus transfer vector and transformed in chemically competent E. coli. A modified colony PCR assay was utilized to identify the positive bacterial colonies that harbor the recombinant plasmid...

A New Whitefly Transmitted Geminivirus Infecting Tomato Plants in Egypt

A-New-Whitefly-Transmitted-Geminivirus

...ular weight of the viral capsids was estimated to be 28 KDa for TYMV. Serological analysis also proved that the putative TYMV belongs to whitefly- transmitted geminivirus by ELISA analysis using the whitefly transmitted-geminivirus (WTG) antiserum [immunogenic oligopeptides coat protein (Cp-3)]. Based on the previous results, the isolated virus TYMV is confirmed to be a whitefly transmitted geminivirus belonging to genus Begomovirus or the Geminiviridae family...

Potential Role of Recombinant Capsid Protein of Dengue Virus Serotype 2 in the Development of an In-House Elisa Based Detection of Infection

Rakhtasha Munir¹, Shazia Rafique²*, Iram Amin^1,2, Sameen Ahmed², Ayesha Vajeeha², Amjad Ali³, Muhammad Shahid², Muhammad Umer Khan⁴ and Muhammad Idrees²

Pakistan Journal of Zoology

Featuring

Morphometric Measurements, Gonopod and Gonopores Appearances in Early Sexual Crablet Stage of Scylla paramamosain (Estampador, 1949)

Muhammad Nur Syafaat, Che Zulkifli Che-Ismail, Adnan Amin-Safwan, Mohamad N. Azra, Mohammad Syahnon, Ambok Bolong Abol-Munafi and Mhd Ikhwanuddin

Pakistan J. Zool., Vol. 57, Iss. 2, pp. 759-766
Preparation of High Selenized Gastrodia elata Blume Polysaccharide and its Immunomodulatory Effects on RAW264.7 Cells and Cyclophosphamide-Treated Mice

Fengwei Ma, Qihua Wen, Qingfang Deng, Yihao Lu, Buyan Zhang, Yongyou Cheng and Su Xu

Pakistan J. Zool., Vol. 57, Iss. 2, pp. 747-758
Freshwater Fish Biodiversity in Liangzi Lake

Ke Li, Hai Liu, Jiangang Zhou, Hongbing Chen and Zhengxiang Wang

Pakistan J. Zool., Vol. 57, Iss. 2, pp. 737-745
Histopathological and Molecular Analyses of Leptospira borgpetersenii and Leptospira interrogans in Bovine Kidneys in Kota Bharu, Kelantan, Malaysia

Intan Noor Aina Kamaruzaman, Siew Zee Ong, Iman Natasha Sofea Jafri, Dayangku Umi Aqilah Pengiran-Isa, Mohamad Sabri Abdul-Rahman, Mohd Farhan Hanif Reduan, Thilini Nisansala, Soon Heng Goh, Siti Nur Haslina Mamat, C.W. Salma C.W. Zalati, Sazaly AbuBakar and Shih Keng Loong

Pakistan J. Zool., Vol. 57, Iss. 2, pp. 727-736