Faiza Ambreen*1 and Muhammad Javed2
...cide mixture (endosulfan+chlorpyrifos) in peripheral blood erythrocytes of freshwater fish, Oreochromis niloticus by using Comet assay. The 96-h LC50 value of pesticide mixture was estimated for 180-old fingerlings of O. niloticusin a static system and then four sub-lethal concentrations viz. 1/3rd of LC50, 1/4th of LC50, 1/5th of LC50 and 1/6th of LC
Muhammad Khalid Mukhtar1,*, Hummaira Iqbal1, Hafiz Muhammad Tahir2, Haseena Ghulam Muhammad1 and Muhammad Irfan1
...i.e., bifenthrin and chlorpyrifos, on the consumption rate of web weaving spider Cyrtophora citricola, was observed. Live spiders for the study were collected from citrus orchards of Sargodha and maintained individually in the laboratory. Both the control and insecticide treated spiders were offered larvae of Drosophila melanogaster in different densities. C. citricola increased prey consumption as the prey density was increased. Insec...
Inzimamul Haq1*, Shahid Sattar1, Bashir Ahmed1, Qamar Zeb2 and Amjad Usman3
...rasitism was recorded in chlorpyrifos treated plots. We conclude from this experiment that for the management of C. partellus, Imidacloprid is recommended as it exhibited high level of selectivity for T. chilonis parasitization capacity. However, further investigation is needed to study the effect of these chemicals on the whole life cycle of T. chilonis. ...
Shehzad Ghayyur1,3, Sadia Tabassum1, Munawar Saleem Ahmad2, Naveed Akhtar1 and Muhammad Fiaz Khan1,*
...25, 0.75 and 1.25ppb) of chlorpyrifos administered for 96 h has been studied on hematological and seral biochemical components of Oreochromis mossambicus. RBCs, Hb and Hct decrease significantly (p < 0.05) indicating an anemic condition, while the WBCs and platelet counts increased significantly (p < 0.05) in all the treated groups compared to the control group. Among the seral biochemical parameters, the level of blood glucose, cortisol and cholesterol ...
Huma Naz1,*, Sajid Abdullah2, Khalid Abbas2, Wardah Hassan3, Moazama Batool4, Shakeela Perveen5, Sadia Maalik4 and Sajida Mushtaq4
...an) and organophosphate (chlorpyrifos) investigated on the activity of antioxidant enzymes viz. superoxide dismutase, peroxidase, catalase and glutathione S-transferase in different organs (liver, gills, kidney, brain, heart and muscle) of fish, Labeo rohita. The LC50 of chlorpyrifos+endosulfan mixture was calculated as 1.95±0.02 μgL-1 for 96 h with the 95% confidence limits. The fish e...
Ailing Huang, Lingyu Meng, Wei Zhang, Junyan Liu, Guangyao Li, Huihua Tan, Wen Lu and Xialin Zheng*
...cypermethrin, abamectin, chlorpyrifos, thiamethoxam and bisultap on toxicity against 1st-6th instar larvae in Phauda flammans Walker (Lepidoptera: Zygaenidae) were tested using the leaf-dipping method. Meanwhile, effects of these pesticides on detoxifying and protective enzymes in P. flammans larvae were also measured. Results showed that the LC50 value of beta-cypermethrin, abamectin, chl...
Huma Naz1*, Sajid Abdullah2, Khalid Abbas2, Muhammad Rizwan Tariq3, Laiba Shafique4, Ghazala Nazeer5
... hr) of endosulfan (END)+chlorpyrifos (CPF) mixture for Catla catla was computed. The genotoxicity of END+CPF mixture in RBCs of fish was also evaluated by comet assay. To check the genotoxicity of END+CPF mixture, blood of fish was sampled after 24-hr of intervals. Some fishes were also kept in clean water known as negative control (NC) and for positive control (PC) cyclophosphamide was injected into fish. The tolerance limits of C. catla agains...
Mahnoor Pervez and Farkhanda Manzoor*
...dacloprid, deltamethrin, chlorpyrifos, fipronil, thiamethoxam, carbaryl, profenophos and bifenthrin). Among a total of 100 pollen samples, 47% were found to be positive. The most frequently found residues were imidacloprid (11%), thiamethoxam (7%) and carbaryl (6%). Among all 100 nectar samples, 36% samples were positive and most abundant pesticides in nectar were imidacloprid (8%), thiamethoxam (6%) and fipronil (5%). In pollen samples, the residual level of ...
Qaisra Siddique1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3
...icro;gL-1) of chlorpyrifos. Fish was kept under chlorpyrifos stress for two months and samples were collected on weekly basis. It was noted that GST level varied significantly with duration. The GST level was raised in first 28 days after that it was dropped off up to 56-day. The trend of GST in fish tissues was observed as muscle<heart<brain<kidney<gills< liver. However, TPCs in selected tissues of...
Tehreem Usman1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3 and Qaisra Siddique1
...ides viz. endosulfan(ES)+chlorpyrifos(CPF) and endosulfan(ES)+bifenthrin (BIF). The fish behaviour was observed from both treated and control groups. Fish under exposure of insecticides mixtures exhibit abnormal behaviour and tried to jump out from water, come to surface and gulp air, showed increased opercular movement and erratic movements, hyper-activity and swimming very fast. Various tissues such as hepatic, neural, renal, cardiac, gills and muscle tissue...
Anum Feroz1, Abdul Rauf Shakoori2 and Farah Rauf Shakoori1*
...20) of bifenthrin, chlorpyrifos and their combinations was studied on the specific activities of esterases in the 4th and 6th instar larvae of susceptible laboratory strain (Lab-S) and deltamethrin resistant population (GUW) of stored grain pest, Trogoderma granarium. After exposure to LC20 of bifenthrin, chlorpyrifos and their combinations of 3:1 and 1:3, the activities o...
Rahat Afza, Muhammad Asam Riaz*, Muhammad Afzal and Muhammad Zeeshan Majeed
Adverse Effect of Sublethal Concentrations of Insecticides on the Biological Parameters and Functional Response of Predatory Beetle Coccinella septempunctata (Coleoptera: Coccinellidae) of Brassica Aphid
...cloprid, profenophos and chlorpyrifos treated aphids altered the functional response of larval coccinellids from type II to III. The change in response was linked to unconsciousness and disorientation induced by the insecticides targeting insect nervous system. Conclusively, none of the insecticides was found safe and all insecticides have had adverse effects on the life parameters of coccinellid beetle C. septempunctata. However, thiamethoxam, lambda-cyhaloth...
Nafisa Shoaib* and Aisha Majid Ali
...anophosphate pesticides (chlorpyrifos, malathion), synthetic pyrethroid pesticide (cypermethrin, lambda-cyhalothrin) and herbicide (buctril). Haemocytes of bivalve play an important role in immune defense and detoxification of contaminants. The bivalves were exposed to different concentrations of test pesticides in a static system. The MN frequencies of all the pesticide treated mussel groups increased significantly (p< 0.05) until the end of the exposure p...
Muhammad Amin1*, Masarrat Yousuf1 and Naveed Ahmad2
...on malathion (1.42 ppm), chlorpyrifos (0.125 ppm) and lambda-cyhalothrin ( 0.0039 ppm) for 24 and 48 h and then total protein was estimated in the brain, gills and muscle tissues. Significant (p< 0.005- 0.00) decreased in total protein was noted in response to all the pesticides as compared to control group and a slight increase was also noted during 48 h as compared to 24 h. The level of total protein was decreased for pesticides in order of
Qaisra Siddique1*, Sajid Abdullah1, Huma Naz2, Khalid Abbas1, Laiba Shafique1 and Qingyou Liu3
.../6th of LC50) effects of chlorpyrifos (CPF) on biochemical markers such as glutathione S-transferase (GST) activity and total protein contents in various tissues (gills, hepatic, renal, brain, muscle and cardiac) of Labeo rohita was determined. Fish was exposed for 60-day and sampling was done after 7-day. Results showed that the GST activity was considerably increased in L. rohita as compared to control. The GST activity was enhanced in various tissues (hepat...
Bilal Jan1, Farida Anjum1*, Aasma Bibi1, Syed Roohul Hussain1, Abdul Ahad1, Azeem ud Deen1, Zia Ullah2 and Farrakh Mehboob3
...azine, parathion-methyl, chlorpyrifos, captan, α-endosulfan, dieldrin, β-endosulfan, endosulfan sulfate, and α-cypermethrin) of various chemical classes, using Agilent’s Intuvo-9000 GC-µECD system. A total of thirteen peach samples, ten from fruit orchards and three from fruit markets, belonging to five different varieties were collected and analysed. 38% of the samples were found to be non-compliant to EU-MRL for
Marwa S. Khattab1*, Ahmed H. Osman1, Huda O. AbuBakr2, Rehab A. Azouz3, Asmaa A. Azouz4, Heba S. Farag5
Hina Mumtaz, Muhammad Zeeshan Majeed*, Muhammad Afzal, Muhammad Arshad, Arif Mehmood and Muhammad Qasim
...0L (52.0–100%) and chlorpyrifos @ 1000 mL/100L (52.0–98.0%) showed significantly (P < 0.05) higher mortality than other chemicals. Higher concentrations of abamectin (6000 ppm), chlorpyrifos (12000 ppm) and chlorantraniliprole (700 ppm) showed 100% larval mortality at 24 h post-exposure. Lambda-cyhalothrin (3500 ppm) showed 95% larval mortality at 24 h and 100% mortality at 48 h of application. The medium dose...
Naila Amjad1, Muhammad Khalid Mukhtar1*, Hafiz Azhar Ali Khan2, Shafaat Yar Khan1 and Sania Waliat1
...nd 10.70-18.81 folds for chlorpyrifos. The RR values at LC50 for imidacloprid and pyriproxyfen ranged from 4.35-28.0 and 10.56-21.45 folds, respectively. The study showed varying levels of resistance in M. domestica populations from area to area and from insecticide to insecticide. Therefore, to control resistance development in M. domestica from livestock facilities, inappropriate and excessive use of insecticides must be controlled through proper mechanisms ...
ADEEBA SYED1, DILAWAR HUSSAIN2, UZMA RAFI1 & SUMAIRA MAZHAR1*
...c exposure of pesticide, chlorpyrifos (CPF) to the fresh water fish Labeo rohita. During acute exposure, fish were exposed to different concentrations of CPF ranging from 0, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03, 0.04 and 0.05 mg/L for 96 hrs in glass aquaria. The 96 hrs LC50 value of CPF for Labeo rohita was found to be 0.01 mg/L. During chronic exposure fish were subjected to 1/3rd, 1/5th, 1/7th and 1/9th of LC50 for 30 days. At the end of the ...
Huma Naz1*, Sajid Abdullah2, Tanveer Ahmed3*, Khalid Abbas2, Syed Qaswar Ali Shah1, Muhammad Rizwan1, Fareeha Latif4, Adnan Ahmad Qazi1, Muhammad Adeel Hassan5
...rohita after exposure to chlorpyrifos+endosulfan (CPF+END) by using Comet and Micronucleus Assay. Twenty fingerlings of L. rohita was kept in LC50 concentration of CPF+END, (1.95±0.02 μgL-1 for 96 h) for 5 days after acclimatization. The blood sample of 5 fish was collected after 24, 48, 72 and 96 h to see the DNA damage and nuclear anomalies. Results of the current study revealed that damaged nuclei (DN%) and genetic damage index (GDI) in RBCs of L....
Muhammad Qasim1, Muhammad Zeeshan Majeed1*, Muhammad Arshad1, Umair Abbas1, Mehar Zubair Shehzad1 and Abu Bakar Muhammad Raza1,2
...ortality was recorded by chlorpyrifos (100.0%) and fipronil (95.0%) at 72 h post-exposure with minimum LC50 values of 1.29 and 2.04%, respectively. Similar trend of effectiveness was exhibited by their LT50 values. Minimum mortality of C. heimi workers was recorded by the formulations of chlorantraniliprole and abamectin. Based on overall study results, it is concluded that chlorpyrifos and fipronil are effective synthetic t...
Huma Naz1*, Sajid Abdullah2, Fariha Latif3, Farzana Abbaasi4, Naila Hadayat5, Tanveer Ahmed6*, Khalid Abbas2 and Muhammad Adeel Hassan7
...al effects of endosulfan+chlorpyrifos mixture on biochemical parameters viz. peroxidase (POx), superoxide dismutase (SOD), catalase (CAT) and Phase-II glutathione (GST) in hepatic, neural, nephron, bronchial, cardiac and muscle tissue of Catla catla and Labeo rohita. The geno-toxic effects were studied in terms of DNA damage and nuclear abnormalities in the RBCs of both fish species. The fish was exposed to the mixture for 60 days and the fish sample was taken...
Pakistan Journal of Zoology
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