Sub-lethal Effects of Chlorpyrifos on Glutathione S-Transferase Activity and Total Protein Contents of Fish, Labeo rohita
Sub-lethal Effects of Chlorpyrifos on Glutathione S-Transferase Activity and Total Protein Contents of Fish, Labeo rohita
Qaisra Siddique1*, Sajid Abdullah1, Huma Naz2, Khalid Abbas1, Laiba Shafique1 and Qingyou Liu3
ABSTRACT
In this work, sub-lethal (1/3rd, 1/5th and 1/6th of LC50) effects of chlorpyrifos (CPF) on biochemical markers such as glutathione S-transferase (GST) activity and total protein contents in various tissues (gills, hepatic, renal, brain, muscle and cardiac) of Labeo rohita was determined. Fish was exposed for 60-day and sampling was done after 7-day. Results showed that the GST activity was considerably increased in L. rohita as compared to control. The GST activity was enhanced in various tissues (hepatic, brain, cardiac, gills, renal and muscle) of CPF treated fish as compared to control group. Comparison among different concentrations indicated that 1/3rd of LC50 caused greater increase in GST activity followed by that of 1/5th and 1/6th. The GST activity in selected tissues of fish varied with duration of exposure as 28>21>35>42>14>7>49>56. Total protein contents in all selected tissues of CPF exposed fish were decreased with the passage of time. Among all the concentrations, 1/3rd of LC50 cause greater decrease in protein contents. The total protein contents in various tissues of L. rohita followed the order: muscle>hepatic>brain>gills>renal>cardiac.
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