Search for Epigenetic

Molecular Effects of Polymorphism in the 3'UTR of Osteopontin Gene in Riverine Buffalo

Sidra Manzoor¹,Asif Nadeem^1,*, Masroor Ellahi Babar², Wasim Shehzad¹, Abu Saeed Hashmi¹, Muhammad Imran¹, Tanveer Hussain², Abdul Wajid¹ and Maryam Javed¹

... due to environmental or epigenetic effect, sampling number or a breed difference. To know the functional consequences of the identified polymorphism, RNA secondary structure analysis was performed and SNP was found changing the termination loop shape in mRNA. However, to increase the selection efficacy of dairy animals, the effects of candidate polymorphism should further be screened in different environments and genetic backgrounds.

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HSPC117 Improves the Rate of Embryonic Development by Up-Regulating the Transcription of Proto-Oncogenes

Hong Ma*, Bo Fu, Liang Wang, Zhong-qiu Li and Di Liu*

..., and can be modified by epigenetics. However, whether HSPC117 affects development of cloned embryos mRNA expression is unknown. To investigate the influences of HSPC117 on embryonic development, we generated transgenic porcine embryos by handmade cloning. We then assessed the embryonic developmental rate at cleavage and blastocyst stages. Our results showed that the HSPC117 transgenic embryos had markedly higher cleavage and blastocyst ra...

Epigenetics: A Bridge between Artificial Light at Night and Breast Cancer

Hafiza Sadaf Zahra¹, Asia Iqbal², Sayyeda Hira Hassan¹, Hafiz Abdullah Shakir¹*, Muhammad Khan¹*, Muhammad Irfan³, Chaman Ara¹, Shaukat Ali⁴

...hich results in abnormal epigenetic changes that relates with an increased risk of BC. The most important ALAN-mediated epigenetic changes include methylation of DNA and acetylation of histone, which are significant for growth, development and progression of BC. DNA hypermethylation of promoter CpG islands inhibits transcriptional activity by methyltransferase enzyme which results in inactivation of tumor suppressor genes (T...

Analysis of Genomic DNA Methylation in Six Tissues of the Greater Horseshoe Bat (Rhinolophus ferrumequinum)

Sen Liu^1,2, Yue Zhu², Haixia Leng², Ying Wang¹, Xiangju Yin¹ and Yajun Zhao¹*

...on. In previous studies, epigenetic variation in the muscles of bat populations were explored; however, the extent of the patterns and levels of genomic DNA methylation among tissues in chiropteran animals have yet to be investigated. In this study, the epigenetic variation of six tissues in normal state Rhinolophus ferrumequinum (i.e., no pregnancy, nursing, or hibernation) was explored using the F-MSAP technique, in...

DNA Methylation Profile Associated with Fertility Trait in Goat Using Whole-Genome Bisulfite Sequencing

Othman E. Othman1*, Lingjiang Min2, Amira M. Nowier3

...igned to investigate the epigenetic regulation of ovulation rate through DNA methylation profile using WGBS. The whole genome DNA was extracted from ovaries of Zaraibi goats belonging to two different fertility groups: high (HFG) and low (LFG) fertility groups. The extracted DNA was subjected to WGS after treatment with bisulfite. The findings declared that a small difference in the DNA methylation levels is present among high and low fertility groups. The met...

Differential DNA Methylation of Growth Factors in Antlers of Sika Deer and Reindeer

Jian-Cheng Zhai^1,2, Sheng-Nan Wang^1,3, Qiang-Hui Wang¹, Yan-Ling Xia¹, Wei-Shi Liu¹, Ya-Jie Yin⁴ and He-Ping Li¹*

.... To investigate whether epigenetic regulation of the growth factors is important in growth of sika deer, female and male reindeer antlers, methylation status were evaluated using bisulfite sequencing PCR (BSP). The 5´ flanking regions of IGF1, KGF and NGF were cloned from reindeer and sika deer antlers, and the lengths were 2089bp/2107bp, 1474bp/1474bp and 865bp/865bp, respectively. Based on bioinformatics analysis, the regions of IGF1 (-50bp ~ +615bp),...

Effect of DNA Methylation of T Cells on the Development of Experimental Asthma

Xiaoying Liang^1,2, Xiaofang Jiang³, Honglin Jia¹, Ru Zhang¹, Li Gao⁴* and Qing Yang⁵*

Co-Treatment of Caffeic Acid Phenethyl Ester with Chitosan Nanoparticles Inhibits DNA Methylation in HepG2 Cells

Faisal Alzahani¹* and Mohammed Abu El-Magd²*

... studied but the precise epigenetic molecular mechanisms for the combined therapy are still unclear. This study aimed to investigate the epigenetic mechanism of CAPE and/or CNPs on human HepG2 cells. The results revealed a significantly higher cytotoxic effect for CAPE on HepG2 cells than CNPs. The combined therapy with CAPE and CNPs exhibited significantly higher expression of the apoptotic Bax gene and lower expression of ...

Evolution of super-drug resistant microbial strains: mechanisms and strategies for containment

SHEIKH AJAZ RASOOL1, MUHAMMAD SALMAN RASOOL2 & MUNAZZA AJAZ3

...e neuro-diseases). These epigenetical agents have been a source of concern for our planet regarding food safety issues (e.g. infected meat). No doubt, man has made significant achievements in effective and neo-antimicrobials research, one wonders why not a single infectious agent has been completely knocked out. Our group has been focusing on ascertaining the basis of antibiotic deferring processes acquired by (indigenous) clinical strains. Accordingly, sub-le...

Precision Gene Regulation Exploiting CRISPR-Cas for Targeted Epigenetic Modifications

Yasameen Waleed Al-Abedi¹, Murtadha Kadhim Hasan²*, Alaa Abdalhadi Halboti³, Ali Abdulhussein Saleh Alsaeedi³, Rafed Abbas Kadhum¹

...rrying out site-specific epigenetic alterations and so uncovering the capability of its use for the upcoming revolution of genetic engineering. The study provides an outline of the CRISPR-Cas system, followed by the mechanisms of genome modification, mainly focusing on the repair mechanisms of the double-stranded breaks. This key idea becomes the starting point of the investigation for the grander pathway that leads to exploring the essence of