Chicken Embryo Fibroblasts are Effective Cells to Study the Function of Chicken Neurexophilin 1 Gene
Dongmei Sun, Meiling Chen, Hafiz Ishfaq Ahmad, Yun Liu, Xunping Jiang and Guiqiong Liu*
...junction, chicken embryo fibroblasts (DF-1 cells) and Hela cells. The immunocytochemistry results showed that neurexophilin 1 was expressed in both the epithelial cells of chicken genital tract and DF-1 cells. Immunofluorescence revealed that neurexophilin 1 was expressed in cytoplasm as well as cell nucleus of chicken fibroblasts and Hela cells. Western-blot analysis displayed neurexophilin 1 in the cell and cell culture fl...
Yang Liu1,2,3, Baimei Liu1,2,3, Chenchen Li1,2,3 and Meiwen An1,2,3,*
...haracterized by aberrant fibroblast phenotypes and excessive deposition of extracellular matrix. Pressure therapy is a common treatment to cure HS, which can regulate collagen synthesis and deposition. To investigate collagen metabolism in keratinocytes and fibroblasts co-culture under pressure, and study the role of IL-1α and MMP-3 in terms of regulating fibroblasts-keratinocytes collagen metabolism with pressure.Chitosan-gelatin scaffolds were fabric...
Muhammad Shahid Nadeem*, Maryam A. Al-Ghamdi and Jalaluddin Azam Khan
...sing non-cancerous human fibroblast cells (FB) and hepatocellular carcinoma cells (HepG2). A dose of 5U/ml of enzyme has shown an anti-proliferative effect up to 65 percent on HepG2 cells.
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Larysa Kladnytska1, Anatoliy Mazurkevych1, Natalia Bezdieniezhnykh2, Oleg Melnyc1, Sergiy Velychko3, Mykola Malyuk1, Vasyl Danilov1, Yuriy Kharkevych1 and Magdalena Gryzinska4*
... by an almost homogenous fibroblast-like cells оn the IVth and Хth passages. It was difined, that fibroblast-like cells оn the IVth and Хth passages was expressed cytoplasmic and membrane proteins, which are specific to proliferating cells. Proliferation index in adipose-derived stem cells culture was significantly greater at the IVth passage – 1.90±0.02...
Feng-Mei Yang1, Rui Li2, Xiu-Xue Hu3, Yong Liang4, Bo Gao3* and Wei Chen3*
... after normal human skin fibroblasts (NHSFs) irradiated by different UVB doses as well as durations, thus unveiling mechanisms underlying HSF senescence induced by Ultraviolet B (UVB). NHSFs treated under different doses (100, 200, 300 mJ/cm2) of UVB with different durations (1, 2, 3 days) were case group, while those without UVB irradiation were control group. Expressions of gene/protein were obtained with real-time quantitative PCR (RT-qPCR) and w...
Shikang Deng1,2, Yan Jin2, Jing Xu2, Xiufang Zhu2, Pinghai Hu2, Jiao Li2, Li Zhang2 and Jianzhong Tang2,*
...1, cyclinD1, and CDK4 in fibroblasts of miniature pig bile duct scar model cultured in vitro. The bile duct scar fibroblasts were replicated by incision and anastomosis in miniature pigs and bile duct scar fibroblasts were obtained. After cell identification, the bile duct scar fibroblasts were given different concentrations of 5-FU (0, 0.001, 0.01, and ...
Somia Shehzadi1, Sana Javaid Awan2, Maryam Javed1*, Asif Nadeem1 and Tahir Yaqub3
...f equine Achilles tendon fibroblasts (ATFs) is a feasible method to study hallmarks of tendinopathy, but ATFs should be viable and grown enough. For this purpose proper medium and supplements are required, which contain suitable growth factors for viability of ATFs. Fetal bovine serum (FBS) is used extensively in cell culture but being expensive and scarce in availability, its alternatives are needed. This study proposed autologous equine serum (AES) and adult...
Hesham M. Abd El Halim1, Maysa M. Hegazy1,2, Aishah Alatawi3, Imtiaz Ali Khan4 and Munawar Saleem Ahmad5*
...nes (SFch-1, SFch-2) and fibroblast growth factor receptor gene (SFbtl) were demonstrated. Cell sensitivity assay (MTT) showed that golden piller was more toxic than galangal in cell viability tests and more potent in the cell growth inhibition. The calculated LC50 were 120 µM and 200 µM for golden piller and galangal, respectively. Both plant extracts significantly reduced the gene expression levels of chitinase genes and SFbtl (p<0....
Bingjie Zhou1, Hitesh Bhagavanbhai Mangukiya1, Siva Bharath Merugu1, Fakhar-un-Nisa Yunus1, Yuchen Fan1, Zhenghua Wu1,* and Dawei Li1,2,*
...y its internalization in fibroblasts. Therefore, the purified recombinant fluorescent protein would be a very useful tool for further investigation of AGR2’s extracellular function and molecular mechanism.
...
Niaz Muhammad1, Samina Yasin1, Zunaira Fatima1, Noor ul Ain1,
Muhammad Faizan2 and Sadaf Naz1*
Xiaoguang Su1,*, Yanjun Gao2, Yanling Wang1 and Yaohui Ma2
... and apoptosis of keloid fibroblasts and its mechanism. Primary HFB cells were isolated and cultured, and treated with different concentrations of HAE for 48 h. Cell viability was measured by MTT. Flow cytometry was used to detect the apoptosis rate. qRT-PCR and Western blot were used to detect the effects of HAE on miR-128-3p and EPHB2. The above method was used to detect cell viability and apoptosis rate after overexpression of miR-128-3p or inhibition of EP...
Afifa Yaqub1, Mehroze Amin1, Qindeel Fatima1, Rabail Hassan Toor1,3, Saira Aftab1 and Abdul Rauf Shakoori1,2*
.... The gene expression of fibroblast growth factor 1 (FGF1), enolase 2 (ENO2), cyclin D1 (CCND1), catalytic subunit of AMP-activated protein kinase (AMPKα2), RNA polymerase II transcription elongation factor (ELL2), and pro-apoptotic Bcl-2 homology 3-only protein (BIM) in MCF-7 breast cancer and normal human embryonic kidney HEK-293 cell lines were studied in response to treatment with different concentrations of UNC0642. Inhibition of G9a expression was ...
Lei Xu1, Qin Yang2 and XiaoYan Zhang3,*
...to measure the levels of fibroblast growth factor-23 (FGF-23), 25(OH)D and sclerosin in the serum of patients. The levels of sclerosin and FGF-23 in the serum of patients in the experiment group were much higher than those in the control group, while the level of 25(OH)D was lower (P < 0.05). Aggravation of calcification came up with the increases in the levels of sclerosin, FGF-23, intact parathyroid hormone (iPTH), phosphorus, creatinine and uric acid, sy...
Dhiya Altememy1, Mohammad Darvishi2*, Samira Shokri3, Saber Abbaszadeh4
...is rate, and division of fibroblast cells and Masson’s trichrome slides were used to determine the rate of collagenization, wound, and extracellular matrix. After the treatment, the size of the wound in the Tio2 N.P. synthesis by Origanum vulgare, Tio2 N.P. synthesis by carvacrol, methicillin, Tio2 N.P., and alginate pad groups of diabetic rats and alginate pad group of non-diabetic rats were determined 7.03 ± 1, 11.12 ± 1, 10.72 ± 1...
Hesham A. Mahran1,2*, Shawky M Aboelhadid3, Saad S. Alqahtani4,5, Mohamed Z. Sayed Ahmed4,5
...afer for human skin cell fibroblast and human epidermoid Skin carcinoma than the other forms at high concentrations. Meanwhile, CNE was potent antibacterial activity even at the lowest concentrations. Moreover, CNE showed better pupicidal activity than the other forms with LC50 at 322 ug/mL lower than pure cinnamon oil, 465 ug/mL against pupae. Additionally, the CNE achieved the lowest concentration after 90 min (15.80ug/mL). The high concentration of 69.60ug/...
Zongliang Li1, Rongxin Ma2,*, Yan Wei1 and Jianghua Zuo3
... growth factor and basic fibroblast growth factor in children with cutaneous hemangioma. Eighty children with hemangioma treated in the Provincial Women and Children’s Hospital from May 2018 to April 2019 were enrolled in the study, including 37 males and 43 females. The children were randomized into two groups according to the experiment: the conventional treatment group (taking one tablet of propranolol) and the combination treatment group (topical app...
Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3
...li cattle embryos, Basic fibroblast growth factor (FGF2), Epidermal growth factor (EGF), In vitro culture
...
Hui Fang*, Kai Xue and Teng Yang
... may positively regulate fibroblast migration, calcium signaling, and cell division based on GO and KEGG analysis. What’s more, by detecting the expression of the key ADPKD-related miRNA-mRNA in vitro experiments, two miRNAs (miR-199a-3p, miR-107) and four candidate target mRNAs (Protein Kinase C Epsilon, PRKCE; Transmembrane P24 Trafficking Protein 5, TMED5; RUNX1 Partner Transcriptional Co-Repressor 1, RUNX1T1; and Fibroblast
Ahmed Abd El-Samie H. Ali
...pression were evaluated. fibroblast and peripheral blood mononuclear (PBMC) cells were in vitro infected and stained by direct double staining with both human and bovine anti-MHC class I and II antibodies labeled with phycoerythrin (pink) 1:20 and anti-BVDV polyclonal antibodies labeled with fluorescein isothiocyanate. FITC (green) 1:60. There was a reduction in the percentages of infected fibroblasts and PBMC expressing bot...
Rawaa Saladdin Jumaa
...ages. The chicken embryo fibroblast (CEF) cells propagated by the prepared infected CAM have shown the cytopathic effect (CPE) that included rounding, detachment of the cells from the monolayer and aggregation of cells during the first and second tissue culture passages. The results of sequencing analysis of pigeon poxvirus field samples revealed that the gene has some mutations (nonsense and missense) and is highly conserved among pigeon poxvirus isolates. Th...
Muhammad Talha Sajjad1, Hamid Akbar1*, Muhammad Arif Khan1, Muhammad Hassan Mushtaq3, Shehla Gul Bokhari1, Muhammad Abid Hayat4 and Ghulam Mustafa2
...zation and the number of fibroblasts were assessed by cytokeratin staining. All data were statistically analyzed. We observed that PRP-wounds had highly significantly (P<0.01) increased levels of neovascularization on days 14 and 28 than control wounds. PRP-wounds had highly significant (P<0.01) increases in re-epithelialization levels and fibroblast numbers at days 14 and 28 than control wounds. In conclusion, PRP-tre...
Shumaila Usman1,2, Irfan Khan2, Kanwal Haneef3 and Asmat Salim2*
...iation ability of mature fibroblasts by using transgene free cellular approach in order to improve the cell-based therapeutics for diabetes. In the present study, we analyzed the effect of 2, 4 - Dinitrophenol (DNP) on the transdifferentiation of NIH3T3 cells into insulin producing β-cells (IPCs). DNP is a lipophilic weak acid that uncouple oxidative phosphorylation by decreasing ATP production. Cells treated with DNP were analyzed for the morphological c...
