Antioxidants Affect Heat Stress Proteins Reactivity on Dermal Fibroblast Cells of Buffalo In Vitro
Antioxidants Affect Heat Stress Proteins Reactivity on Dermal Fibroblast Cells of Buffalo In Vitro
Bipasha Mazumder1, Khadija-Tut-Tahira2, Khondoker Moazzem Hossain1, Gautam Kumar Deb2,3*, Md. Ashadul Alam3, S. M. Jahangir Hossain4
ABSTRACT
High ambient temperatures adversely affect buffalo production and reproduction. Dermal fibroblasts are essential for regulating temperature homeostasis in the skin. This study aimed to evaluate the mRNA expression profile of different heat shock proteins (HSPs) in dermal fibroblast cells due to heat stress and examine the effect of inorganic zinc and vitamin C in this condition. For this study, the primary fibroblast cells of the Murrah Cross were used and identified by FSP1 and FN_1 primers. The pretreated cultured cells with zinc and vitamin C were kept at 42°C for three hours, as buffaloes in tropical areas experience such temperatures in the summer. The culture cells were subsequently allowed to recuperate at 37°C and collected at various time points along with control samples (unstressed), and cellular viability was determined using the trypan blue dye exclusion method. Heat stress significantly (p<0.05) decreased cell viability. Zinc-propagated cells had higher cell viability (p<0.05) and proliferation rate than vitamin C-propagated cells across post-heat stress, representing zinc’s protective nature against heat stress-induced cell apoptosis and cell injury. HSPs expression studies revealed that heat-responsive genes (HSPA1A, HSPA2, HSPA8, and HSP90AA1) were induced after heat stress and remained upregulated during the post-heat stress periods. Among them, the HSPA2 gene displayed maximal induction across post-heat shock periods. Zinc and vitamin C treatments showed a significant (p<0.05) reduction in most HSPs’ expression across different post-heat stress periods, which may be due to their pivotal role in the body’s immune system and oxidative stress management.
Keywords | Dermal fibroblast, Heat stress, Heat shock proteins, Cell viability, Zinc, Vitamin C
To share on other social networks, click on any share button. What are these?
