Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek
...h in-vitro and in-vivo. All fungi proved to be efficient in reducing final population of northern root knot nematode M. hapla and giving vigour to the plants. In laboratory experiment, L. muscarium was the most effective against nematode eggs (95.6%) and second stage juveniles (J2) (95.8%) infection. In greenhouse experiment, similar trend was found. L. muscarium proved to be more effective against M. hapla whereas ...
A. K. Chaubey and Satyandra Kumar
Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
... in PDA media plates. In in-vivo test soil was amended with antagonist and wilt fungal and bacterial isolates 2 x 106 CFU/ml and 2 x 108 CFU/ml respectively as talc formulation @ 0.2% (w/w). Twenty one days old tomato seedlings were transplanted after dip treatment in antagonist fungal and bacterial CF in earthen pots filled with amended soil. Seven days after transplanting, the seedlings were inoculated with second stage juveniles of M. incognita @ 2J2/g soil...
Muhammad Hidayat Rasool*, Abuzar Muhammad Afzal*, Abu Baker Siddique*, Waseem Akram**, Muhammad Saqalein*, Madiha Basit* and Muhammad Farooq Tahir***
ANTIVIRAL ACTIVITY OF VIRO CARE GZ-08 AGAINST NEWCASTLE DISEASE VIRUS IN POULTRY AND ITS IN-VITRO CYTOTOXICITY ASSAY
...d up invitro, in-ovo and in-vivo. The cytotoxicity assay of the product was performed using Vero cell line. All the trials revealed that the stock solution and 1:2 dilution of GZ-08™ had some antiviral activity as well as were cytotoxic. As the concentration decreased, cytotoxicity as well as antiviral activities were lost. Based on these findings, it was concluded that GZ-08™ sanitizer or spray can be used as antiviral agent to clean or disinfect ...
Aneeqa Saleem1, Muhammad Islam1, Hamid Saeed1* and Mehwish Iqtedar2
Misbah Ali1, Safdar Ali1, Muhammad Ahmad Zeshan2*, Rana Binyamin3, Nadeem Ahmed4, Muhammad Usman Ghani5 and Awais Ahmed Khan2
...icola under in-vitro and in-vivo conditions. The poisoned food technique was used for in vitro trials with three concentrations (150 ppm, 200 ppm, and 250 ppm). The most effective concentration of fungicides from laboratory experiment was used in field conditions against red leaf spot of sorghum. Results showed that Cabrio Top was the most effective fungicide by showing 88% fungal inhibition while Native also exhibited good results by showing 78.1% fungal inhi...
Muhammad Mobashar1, Qazi Aqeel1, Muhammad Tahir Khan1, Assar Ali Shah2*, Ahmed A.A. Abdel-Wareth3, Salman Khan4, Nazir Ahmad1, Sami Ullah1 and Haq Amanullah5
..., growth performance, in-vivo and in-saco nutrient degradability in Kari sheep. A total of 36 Kari sheep were randomly weighed and offered the following four type of ration: A, total mix ration (TMR) + alfalfa (AL) 70 % and Prangos pabularia (PP) 0 % (control); B, TMR + 24% PP: 46 % AL; C, TMR + 46% PP 24 % AL and D, TMR + 70% PP: AL 0 % on dry matter basis were fed to experimental sheep for a period of 90 days. The dry matter intake, crud...
Soliman Mohammed Soliman
...d methane production, in-vivo rumen fermentation parameters, and milk production in dairy cows. Twenty Friesian cows were randomly allocated to four groups (five cows /group). All cows were fed TMR (total mixed rations). The first group was fed TMR without supplementation, groups from 2 to 4 were fed TMR supplemented with one of the feed additives S. cerevisiae, yucca, or green seaweed at a rate of 25 g, 20 g, and 100 g/head/day for respecti...
Afifa Yaqub1, Mehroze Amin1, Qindeel Fatima1, Rabail Hassan Toor1,3, Saira Aftab1 and Abdul Rauf Shakoori1,2*
...d with G9a, in-vitro and in-vivo safety analyses is required to fully establish G9a as a therapeutic target for cancer progression and UNC0642 as an anti-cancerous therapeutic agent for the treatment of breast cancer. ...
USMAN AHMAD*1, AMTUL JAMIL SAMI1, MADEEHA KARAMAT1, MADEEHA KHALID1, FAIZAN NAEEM2 & MUHAMMAD JAMIL YOUSAF3
... instrument in-vitro and in-vivo. Fickian diffusion mechanisms have been read for the release of insulin at diverse pH (5.4 and 7.5).
Amthal Ahmed Fouad1, Basem Mohamed Ahmed2, Momtaz Abdelhady Shahein1, Hussein Aly Hussein2*
...s RABV particle dictates in-vivo studies. ...
Shaista Ilyas1, Safdar Ali1, Amer Habib1, Misbah Ali1,2, Muhammad Ahmad Zeshan3*, Yasir Iftikhar3, Muhammad Usman Ghani4,5 and Muhammad Umair1
...nation of all these. The in-vivo experiment was carried out in the open field using randomized complete block design (RCBD). Effects of different chemicals on disease management were examined statistically by using Tukey’s honestly significant difference (HSD) test at 5% level of significance. The most effective treatment was the combination of all 3 chemicals that gave maximum disease control and 11.72% disease severity was recorded. Mungbean cultivars ...
Saad Tahir, Nadeem Ahmed*, Mohsin Ahmad Khan, Muhammad Akram, Rabia Abbas and Kausar Malik
...o increase the half-life in-vivo and also invoke less immune response. We designed codon-optimized HSA-SK fusion gene, integrated into -pPICZaB vector, cloned and transferred into Pichia pastoris strain GS115. The transformants showing highest resistance for Zeocin™ was selected for protein expression which produced to >350mg/L HSA-SK fusion protein. Further, affinity chromatography was carried out for the purification of the HSA-SK fusion protein. SD...
Ram Prasad Ghimire
... goats, and a subsequent in-vivo experiment determining the apparent digestibility of fodder nutrients of those browse species. Both of the experiments were conducted in Randomized Complete Block Design (RCBD) with six treatments and five replications on native hill goats of Nepal. The fodders of popular five browse species from the pasturelands of Nepali middle hills; Dhayero (Woodfordia fruticosa Kurz), Musure katus (Castanopsis tribuloides (Sm.) Lindl.)), B...
Deni Novia*, Indri Juliyarsi, Rizki Dwi Setiawan, Clara Mustika, Cindi Melani
...operties. We employed an in-vivo trial with a completely randomized design consisting of five treatments and four replicates. The egg tea was dried using a food dehydrator at 60°C for five different drying times (2, 3, 4, 5, and 6 h). The parameters evaluated were antioxidant activity, energy value, moisture, protein, fat, ash content, solubility, color (L*, a*, b*), and sensory evaluation of hedonic preferences and qualities. The results revealed that dif...
Nawab Ali1, Akeel Ahmed Memon1, Asmatullah kaka1, Amjad Hussain Mirani2, Muhammad Ibrahim Panhwar3*, Nisar Ahmed Solangi1, Kashif Ali Malik1, Fazul U Rahman1, Moin Akhtar Vistro1
...n-thaw sperm traits) and in-vivo conception rate of Kamohri buck semen. ...
Muhammad Shahkar Uzair1, Muhammad Mushtaq1, Muhammad Shuaib1*, Saqib Nawaz2, Mehboob Ali2, Faiz ur Rehman2, Abubakar Sufyan3, Usman Zeb4, Muhammad Ayaz5 and Muhammad Aamir Khan5
...stage. Both in-vitro and in-vivo studies were carried out to evaluate the antimicrobial activities and potency of this combination. A total of one hundred and eighty day-old chicks were randomly allotted to 6 groups i.e. G1 (negative control), G2 (positive control), G3 (standard antibiotic neomycin only), SN-1 (Serratiopeptidase @40g/L+Neomycin@10mg/L), SN-2 (Serratiopeptidase @50mg/L+ Neomycin @20mg/L), and SN-3 (Serratiopeptidase @ 60mg/L+ Neomycin @30mg/L)....
Roy Sukbir Singh1, Jekson Martiar Siahaan2,3*, Endy Juli Anto4, Syafruddin Ilyas5, Putri Eyanoer6, Hendrika Andriani7,8
...loyed both in-silico and in-vivo approaches to explore these effects. The in-silico analysis involved molecular docking simulations to evaluate the interactions of Aloe vera compounds with inflammatory mediators such as TNF-α and COX-2. Furthermore, this study also employed a laboratory design with a post-test randomized controlled group. The study included 30 male white rats divided into six groups. Prior to the study, the rats were induced with a high-...
Asfand Yar Khan1, Syed Saleem Ahmad1*, Muhammad Avais1 and Kamran Ashraf2
...ased on the in-vitro and in-vivo trial. A total of twenty-one (n=21) PCR-confirmed isolates were selected for detection of virulent genes in-vitro trial (zone of inhibition). For in-vivo trial, forty-two (n=42) day-old chicks were divided into seven equal groups randomly (n=6 birds/group), and were classified as: G1 (Azadirachta indica @ 18.25 mg/ml), G2 (Melia azedarach @ 15.75 mg/ml), G3 (Withania coagulans @ 25 mg/ml), G4...
Abdul Qadeer1,2, Muhammad Avais1*, Abdul Wajid3, Muhammad Zahid Iqbal1, Hanif Ullah4, Asad Khan5, Sajid Ur Rahman2,6, Aqeel Javeed7 and Sher Zaman Safi8,9
...lix was assessed for its in-vivo anthelmintic activity at doses of 1.13 and 2.25 gm/kg respectively in naturally infected sheep with H. contortus. ED50 of egg hatch assay (EHA) was studied with a dose of 0.103 for methanolic extract, 0.117 for aqueous extract, and 0.069 for albendazole. ED50 of LDA was seen at a dose rate of 0.035 for methanolic extract, 0.091 for aqueous extract, and 0.058 for albendazole. The in-vivo study...
