Isolation and Identification of Pink-Pigmented Facultative Methylotrophic Bacteria (PPFM) from the North Rumaila Field, Iraq
Isolation and Identification of Pink-Pigmented Facultative Methylotrophic Bacteria (PPFM) from the North Rumaila Field, Iraq
Anwar A. Maki* and Asaad M.R. Al-Taee
ABSTRACT
Petroleum bioremediation is internationally recognized as a cost-effective and ecologically sustainable solution. The aim of this study is isolating and identifying the PPFM bacterium from oil contaminated soil. So, this bacterium was isolated from soil samples collected from Rumaila oil field in Basra, southern Iraq, using a mineral salt medium (MSM) complemented with 1% methanol as the only carbon and energy source. Genetic identification of the promising bacterium was performed using the 16S rDNA gene and identified as Methylorubrum pseudosasae AAZ2 (OR226418.1). MxaF gene that encodes for the methanol dehydrogenase enzyme was also detected, confirming the identification of PPFM. Growth was achieved in MSM medium supplemented with 1% crude oil as a carbon source, recording highest optical density (OD600) of 0.7 after 5 days of incubation in a shaking incubator at 120 rpm and 30 °C. Gas chromatography (GC) analysis showed high rates of aliphatic hydrocarbons (n-alkane) degradation after incubation in the MSM medium with 1% (w/v) crude oil at 65.45% and 74.6% after 5 and 10 days of incubations, respectively. It also showed high rates of poly aromatic hydrocarbons (PAH) degradation of 94.77% and 98.11% after 5 and 10 days of incubation. The Methylorubrum pseudosasae AAZ2 strain has proven to be highly efficient in remediating crude oil, and hence can be exploited to remediate contaminated environments.
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