Xingdong Yang and Lijuan Yuan
...nd normal animals in the maturation status of immune systems caused by the lack of gut microbiota can be minimized by establishing human-gut-microbiota transplanted gnotobiotic pig models. Given the advantages of gnotobiotic pig models, it is expected that they will be used more widely in biomedical research for studies of human viruses and other human diseases....
Muhammad Imran*, Munir Ahmad, Muhammad Naeem, Muhammad Farooq Nasir, Imran Bodlah, Muhammad Nasir and Umer Ayyaz Aslam Sheikh
...tiation, development and maturation stages under controlled laboratory conditions. Queen oviposition rate was 1.4-fold higher in polycarbonate box compared to wooden and cardboard materials. Early start of egg-laying (first oviposition period) and earlier emergence of first worker were found in polycarbonate box as compared to others. Similarly, colony foundation stage in polycarbonate box took 58.3 days which was about 7-10 days shorter than others box types....
Wenqiao Hui1, Jishun Tang1, Dejian Zhu1, Qian Ban2* and Sheng Chen1*
... the process of physical maturation of an animal with the capability of sexual reproduction. In domestic animals, age at attainment of puberty is an event that contributes significantly to lifetime reproductive efficiency. Anhui white goat is a native Chinese breed, with the characters of early time puberty onset and high fecundity. However, the molecular mechanism of puberty onset has not been well understood. It has been demonstrated leptin act as a critical...
Gülnur Metin* and İlker Aydin
...productive season, first maturation size (LM50) and sex ratio) of the jinga shrimp, Metapenaeus affinis (H. Milne Edwards, 1837), in Izmir Bay (Aegean Sea, Turkey) were investigated. A total of 2894 M. affinis were obtained from commercial fishermen between November 2008 and January 2011. The total length and weight of the females or males were measured as 7.3–17.5 cm and 2.6-38.9 g or 7.8–15.4 cm and 2.6-41.6 g, res...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
...ps during the testicular maturation process, however, PDK4, DUSP4 and FKBP1B expression levels were decreased (p<0.01). Furthermore, the luciferase in miR-122/449a co-transfected with pmiR-RB-REPORT-DUSP4-WT or pmiR-RB-REPORT-PDK4/FKBP1B-WT group was significantly lower than pmiR-RB-REPORT-DUSP4-mut or PDK4/FKBP1B-mut and negative control groups. Meanwhile, PDK4, DUSP4 and FKBP1B expressi...
Song Jiang1,2, Fa-lin Zhou3, Jian-hua Huang1, Qi-bin Yang1, Li-shi Yang1 and Shi-gui Jiang1,*
...ntinuously during sexual maturation acceleration, whereas that of the control group fluctuated. At the end of the experiment, the gonadosomatic index (GSI) increased by 607.14% and 100.00% in the treatment and control groups, respectively (P<0.01). The gonadal wet and dry mass ratio (Gd/m) was higher in the treatment group than those in the control group, but the difference was not significant (P>0.05). Furthermore, the bo...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
...e oocyte in vitro maturation and early embryonic development after in vitro fertilization. Sheep follicular fluid (SFF) was collected from different ovaries and supplemented to the in vitro maturation (IVM) media with proportions 0%, 10%, 20% and 40%. Oocyte maturation was assessed in different treatments and then cumulus-oocyte complexes were used for in vitro ...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
...0, which delayed biofilm maturation and even decreased biofilm formation with the passage of time. The micro well cytotoxicity assay against Artemia salina showed the cytotoxicity of the extracts in the range of 10-35%. The chemical profiling of the extracts was done by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC-UV) to get the indication of the identity of the compounds responsible for antimicrobial activity and bio...
Shafaq Fatima*, Sumrin Sahar and Khalid Lone
...ar development and final maturation in June however, spawning in captivity was not observed. Role of P and 17α-HP was identified more as substrate of sex steroids. Higher levels of sex steroids suppressed the synthesis of cortisol presumably interfering with pituitary-inter-renal axis.
...
Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*
...y. After in vitro maturation (IVM) in Tissue Culture Medium-199 (TCM199) for 24 h and fertilization (IVF) in Tyrode’s Albumin Lactate Pyruvate (TALP) medium for about 20 h, the presumptive zygotes were randomly distributed into 6 culture groups; Group I: Synthetic Oviductal Fluid (SOF) medium alone, Group II: SOF + co-culture, Group III: Conditioned SOF, Group IV: M199 alone, Group V: M199 + co-culture and Group VI: Conditioned M199 for in vitr...
Khalid P. Loneand Irfana Liaqat*
...g the first reproductive maturation of Labeo rohita was undertaken. The study was done throughout the age of 18-29 months. For this purpose, nearly 500 fish were kept in ponds and sampled every month for the histology of the testis. After taking data regarding other somatic traits, the testes were fixed in buffered formalin and processed for routine histology and light microscopy. The annual maturity stages can easily be divided into five gross stages n...
Sajida Rasool1, Saba Irshad1*, Neelam Saba1, Mehak Fiaz1, Muhammad Sajid Hussain2, MuhammadWajid Hussain3 and Peter Nürnberg2
... motor neuron growth and maturation. In present study, a Pakistani family of HSP penetrating in autosomal recessive pattern was ascertained. Patients presented spasticity and stiffness of upper and lower limbs, severe microcephaly, dysphagia, no speech, hearing loss and seizures. Genome wide linkage analysis and whole exome sequencing revealed a novel homozygous nonsense mutation (c.204T>G) in BICD2 gene which was predicted to yield a truncated prote...
Lei Gao1, Gong-xue Jia2, Zheng-yuan Huang1, Ming-xing Yue3, Chao Zhang1, Shi-en Zhu1 and Xiang-wei Fu1,*
...) during in vitro maturation (IVM), then the harvested metaphase II (MII) oocytes were parthenogenetically activated to evaluate pronuclear (PN) formation of parthenogenetic embryo and embryo development. The cytoplasmic maturation was examined by measuring the intracellular reactive oxygen species (ROS) and glutathione (GSH). DNA DSBs were examined by immunostaining of pi-H2AX, the marker of DNA DSBs. The results sho...
Fan Sigang1, Guo Yihui1* and Xu Youhou2
...ry mechanisms of gonadal maturation in scallop is critical in the aquacultural industry. Here, gonads in maturing stage were obtained from noble scallops and sequenced using an Illumina high-throughput sequencer, producing 6.68 and 6.70 Gb of data for the ovary and testis, respectively. Reproduction-related genes, including vasa, nanos, and vitellogenin, and sex-determining genes, such as FoxL2, Dmrt, and sox9, were detected. Transcriptome comparison revealed ...
Faiqah Ramzan1,2,*, Irfan Zia Qureshi2 and Muhammad Haris Ramzan3
...mature male mammals, the maturation of gonads is not clear yet. The current study tested the hypothesis that continuous kisspeptin administration suppresses gonadotropin and testosterone release and spermatogenesis. Intraperitoneal kisspeptin administration was carried out in 05 weeks old prepubertal male Sprague Dawley rats, twice daily for 12 days, at three different dosage regimens: 10ρg, 1ηg and 1µg compared to control (saline-treated) rats. ...
Amjed Ali1, Rafi Qamar1*, Muhammad Ehsan Safdar1, Salman Saleem2, Sami Ullah3, Muhammad Arshad Javed4 and Syed Wasim Hasan5
Development and Growth: Influence of Sowing Dates on Performance of Cotton Cultivars
... respectively while boll maturation (48.05) was noted in Bt. MNH886. Sowing date 5th May plot recorded more leaf area index and net assimilation rate in FDH-170 (4.45 and 3.87 g m-2 day-1) while leaf area duration and crop growth rate in Bt. FH-142 (282.32 and 6.67 g m-2 day-1), respectively. Significantly, higher TDM production was recorded on 21st April in Bt. FH-142 (1104.33 g m-2). Therefore, cotton should be grown among after 15th April to before 10th May...
Syahruddin Said1*, Widya Pintaka Bayu Putra1 and Dani Nur Arifin2
...llicular growth, oocytes maturation, ovulation and corpus luteum formation. The aim of this study was to identify the polymorphisms in the exon 11 of bLHR/HhaI gene (SNP g.1553C>T) and its association with service per conception (S/C) of Pasundan cows of West Java, Indonesia. Total of 147 Pasundan cows were used in this study. The PCR-RFLP analysed three genotypes of CC (0.11), CT (0.57) and TT (0.32) were detected in the Pasundan bLHR/HhaI ge...
Riffat Sultana1*, Nuzhat Soomro2, Santosh Kumar3 and Ahmed Ali Samajo2
...uence i.e. age of maturation, duration of pre-copulatory and mating, interval between each mating, length of copulation, and number of mating in entire life of insect and also discussed its implications in the observed mating behavior. We examined that reproductive activity of Oxyinae was at its maximum in August to December. Mating duration has also been identified as a pest control method in which the insect does not become resistant.
...
Xiangzu Wang,1,2 Jifeng Xi,1,2 Bin Jia,1,* Yongsheng Zhang,1 Chaocheng Li1
...lts showed that with the maturation of mouse kidney, the cell proliferation activity decreased, and the expression of ZFX, Bcl-2, and BAX were down regulated. The ZFX gene may participate in the developmental process of the kidney through the balance of cell proliferation and apoptosis regulation.
...
Song Jiang1,2,3, Ming-ge Zhuang1,2,3, Fa-lin Zhou1,2, Qi-bin Yang1, Jian-hua Huang1, Li-shi Yang1 and Shi-gui Jiang1*
..., had the best effect on maturation of parent shrimps. The highest gonad weight increase rate of 1845.28% and the largest consecutive spawning rate with average value of 70.66% were both in groups fed on P. tschiliensis, with no significant difference from the groups fed on N. succinea combination bait. The dynamics of VTG mRNA level were observed in hepatopancreas and ovary of P. monodon with the trend of increasing firstly and then decre...
Miloudi Hilali1*, Hanae El Monfalouti1, Larbi El Hammari2, Farid Amar3, Zakaria Mennane4, Malika Haddam5, Nadia Maata6 and Badr Eddine Kartah1
...8:1 (75.4%). Time of the maturation is a factor that can significantly influence the quality of olive oil. Oleic acid, criterion of nutrition and quality of olive oil. Also, the amount of stearic acid increases with the ripening of the olive fruit. The sterol result shows that the percentage of stigmasterol and β-sitosterol (95.2%) increased with the ripening of the olives. The influence of ripening parameter is significant on the main quality criteria of...
Zhi-Peng Li, Laiba Shafique, Saif ur Rehman, Kui-Qing Cui, Xiao-Can Lei, Xing-Rong Lu, De-Shun Shi* and Qing-You Liu*
...>) during porcine oocyte maturation and early embryonic development were systemically investigated. The expression of Gas6 in the process of follicular development was studied by immunohistochemical staining, immunofluorescence, QRT-PCR and western blot. Immunohistochemical staining results showed that Gas6 was expressed in the oocytes, especially the nucleus, from the primary to preovulatory stages. The further immunofluorescence results showed that
Ali Zohaib1*, Muzzammil Hussain1, Iftikhar Ahmad1, Mushtaq Ali2, Tahira Tabassum3 and Adnan Bashir1
...;">Determination of crop maturation duration, yield and economic benefits of different Basmati rice varieties is imperative for enhancing the output of rice-wheat cropping system. Hence, two-year study was accomplished to find out the differences in growth, crop maturation duration, yield and economic benefits of Basmati rice varieties (Super Basmati, PK-1121 aromatic, Chenab Basmati, Kissan Basmati, Punjab Basmati, Noor Bas...
Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3*, Abd El-Nasser Ahmed Mohammed3
... fluid, effect on oocyte maturation and developmental potential of the resulting embryos in vivo and in vitro. Because of the importance of cumulus cells on follicular development, this review was made to collect and consolidate the current knowledge of cumulus cells effects on maturation of intact and reconstructed oocytes and further developmental competence of embryos in vivo and in vitro.
Keywords | Follicle, Cum...
Anis Suraya Mohamad Abir1, Sakinah Yusof1, Abd Rahaman Yasmin1,2, Abdul Rahman Omar1,3, Kok Lian Ho4, Wen Siang Tan1,5, Abdul Razak Mariatulqabtiah1,6*
...virus induced phenotypic maturation of the cells better than the WT virus. This study supports further understanding of the innate immune response after FWPV infection in chickens.
Keywords | Fowlpox, Avian influenza virus, Chicken, Dendritic cells, Haemagglutinin
...
Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3, Abd El-Nasser Ahmed Mohammed3, Al-Hassan Mohammed4*
...o include oocyte meiotic maturation, fertilization and culture processes. Oocyte maturation is the first step for in vivo or in vitro embryo production. Oocyte maturation appears to be a simple step, but it is a very complex process. Maturation of oocytes include several changes at the cytoplasmic and nuclear levels that makes the oocytes able to undergo...
Abd El-Nasser Ahmed Mohammed*, Aiman Al Mufarji and Sultan Alawaid
...he oocyte for successful maturation, fertilization and further embryonic development. The changes enable the zygotes to overcome maternal zygotic transition stage and follow their developmental competence to fetus. The changes were affected by in vivo and in vitro molecules and factors of the organisms and the surrounding conditions, respectively. Because of the importance of follicular changes during growth and development stages, which reflected in the devel...
Abhishek Pandit1, Suman Poudel2, Manish Gautam3* and Shambhu Shah4
...ts the dominant follicle maturation due to the suppression of both FSH and LH. Gonadotropin-releasing hormone or an analog is administered with PGF2α to estrous- cyclic and noncyclic cattle disrupts the patterns of follicular growth, inducing ovulation on a large follicle. To select and successfully implement the estrus synchronization regime in animals’ proper knowledge of the hormonal profile and functional structure prevalent in ovaries in stage...
W.M.A. El-Nagdi, M.M.A. Youssef† and M.F.M. Eissa
...m to require root tissue maturation
before expressing full resistance against root-knot nematode.
...
Saleh Aljubran, Shaker Al-Suwaiegh, Yousef Alyousef, Sulaiman Alhajri, Mohammed Alghareeb, Abd El-Nasser Ahmed Mohammed*
...ir importance for oocyte maturation, artificial oocyte reprogramming, embryo development and their resulting newborns.
Keywords | In vitro, Oocyte, Embryo production, Embryo transfer, Micromanipulation
...
Chalothon Amporn1, Somchit Guntaprom1*, Sarawut Duongmawong1, Wilasinee Srisanyong1, Sirikanda Thanasuwan1, Phalita Koonnadilokpot1, Dechawut Bunyaluk2, Juggrid Jugsumrit3, Jakrit Yaeram4, Chompunut Lumsangkul5
...ir effects upon in vitro maturation (IVM) media. Experimentally, cumulus-oocyte complexes (COCs) which were obtained from bovine ovaries underwent culturing for a period of 22 h in TCM-199 containing an additional 10% fetal bovine serum, streptomycin (100 μg/ml), penicillin (100 U/ml), 25 μg/ml FSH, 2 IU/ml hCG, 0.2 mM sodium pyruvate, 1 μg/ml 17-β-Estradiol, and either 0, 100, or 200 μM/ml cysteine where 0 served as the control. IVF-TALP medi...
M.A.M.M. Shehab-El-Deen1,2*, S.A. Al-Dobaib1 and K.A. Al-Sobayil1
...fatty acids (NEFAs). The maturation conditions in the current study were resemble the situation in the oocyte microenvironment of metabolically stressed lactating dairy cows during the post-partum period where the negative energy balance occurred. Oocytes were in vitro matured under elevated levels of NEFAs for 24 h in serum-free maturation media. Obtained zygotes were cultured in synthetic oviduct fluid with 5% FCS for eigh...
Beenish Shahid1* and Muhammad Ijaz Khan2
... cytoplasmic and nuclear maturation of in vitro matured oocytes of Nili Ravi buffalo in the presence of hormones. The denuded oocytes (DOs) obtained by repeated pipetting were collected from 2-8mm follicles. A series of experiments were conducted to evaluate the effects of oestradiol (2 μg/ml), recombinant human follicle stimulating hormone (0.05 IU/ml), human chorionic gonadotrophin (2 IU/ml) and insulin (0.12 IU/ml) alone or together in different combinat...
Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3
...EGF in follicular fluid, maturation media and Bali cattle embryo culture media. The study used ovaries that came from slaughterhouse and were oocytes mature before fertilization using semen from one bull, oocytes are fertilized then cultured for 48 hours. Embryo grouping based on cell count was conducted after 48 hours of culture and was recultured for 48 hours. Follicular fluid, oocyte maturation media and embryo culture me...
Pankaj Gargotra1, Judith Betsy C1*, Cheryl Antony2 and Stephen Sampath Kumar J3
...hed diets on the captive maturation and breeding performance of L. thermalis. Vitamins A, C and E were incorporated at three different doses in basal feed containing 23% C.P and fed to brooders for a period of 90 days. Fecundity, GSI, gonad histology, hormone (cortisol and progesterone) variations, breeding performance and growth parameters were recorded. Significant differences in the growth and breeding performance of female brooder under different treatment...
Laila Ibouzine-Dine1*, Inssaf Berkiks1,2, Mouloud Lamtai1*, Hasnaa Mallouk1, Ayoub Rezqaoui1, Abdeljabbar Nassiri1, Abdelhalem Mesfioui1, Aboubaker El Hessni1
...tructural and functional maturation of the central nervous system (CNS). In this study, we focused on the prefrontal cortex, hippocampus, striatum, and hypothalamus of male and female “Wistar” rat pups, as these areas have differential developing states. NO level was determined on postnatal days (PND) 1, 2, 3, 4, 7, 8, 9, 10, 11, 12, 14, 15, 16, and 17. The supernatant nitrite/nitrate (NOx) concentration was assessed after the reduction of nitrates...
Shorouq Mohamed Omara1, Ibrahim Saad El-Shamaa1, Emad Abd-Elaziz Abd-Allah2, Assmaa Abd-Allah Fathy3, Essam-Eldin Tharwat4, Mohammed Hamdy Farouk5* and Ibrahim Mahmoud Abd El-Razek1*
... L-carnitine (LC) on the maturation of Egyptian buffalo oocytes has not been determined yet. This study aims to estimate the effects of supplementing LC on the in-vitro fertilization (IVF) of Egyptian buffaloes. We evaluated blastocyst and apoptosis rates in order to substantiate the potential development of cumulus oocytes–complexes (COC) and determine the effect of LC in reducing the occurrence of apoptosis under in vitro matur...
Ferry Lismanto Syaiful*, Jaswandi Jaswandi, Mangku Mundana, Zaituni Udin
...ch aims to assess oocyte maturation rates, in vitro fertilization, and pronuclei development in Pesisir cattle, and to determine the optimal culture medium and culture duration for in vitro fertilization techniques. The culture media used include TCM-199 medium supplemented with both B-O (Brackett Oliphant) medium (HEPES, without the use of 5% CO2) and modified B-O (mB-O) medium (without HEPES, with 5% CO2). For the oocyte maturation
Pankaj Gargotra1*, C. Judith Betsy1 and J. Stephen Sampath Kumar2
...uo; test. The growth and maturation parameters were analyzed in detail using Regression analysis and compared using one-way ANOVA in SPSS- 22.0....
