Effects of Dietary Tryptophan Supplementation on Melatonin and Oocyte Maturation in African Catfish, Clarias Gariepinus
Effects of Dietary Tryptophan Supplementation on Melatonin and Oocyte Maturation in African Catfish, Clarias Gariepinus
Epro Barades1,4*, Iskandar2, Ibnu Dwi Buwono2, Yuli Andriani2, Ayi Yustiati2, Roffi Grandiosa2, Ratu Siti Aliah3
ABSTRACT
This study aimed to investigate the potential benefits of tryptophan supplementation in feed and its impact on melatonin, estradiol, and vitellogenin levels and oocyte maturation in African catfish. Thirty out of 90 broodstocks, with an average weight of 700 ± 160 g, were randomly selected for treatment, with a male-female ratio of 1:1. A completely randomized design was used with three levels of tryptophan as treatments (0.33% (X), 0.41% (Y), and 0.49% (Z) in dry feed), replicated individually with a total of five broodstocks for each treatment. Broodstock was maintained in the same environment at a constant temperature of 28 °C, pH between 6-7, dissolved oxygen levels of 4-5 mg L-1, and total darkness (24 hours dark). Microchip tags were used for sample identification and replication. Observations and samples were collected from five individuals every 10 days for 30 days. The results showed that tryptophan had an effect (p<0.05) on melatonin, estradiol, vitellogenin, oocyte diameter, egg diameter, yolk diameter, and hatching rate. However, tryptophan content has a non-linear effect especially on egg and yolk diameter, the result showed at X treatment 1.52 ± 0.05 and 1.27 ± 0.04 mm, and Z treatment 1.53 ± 0.02 and 1.27 ± 0.02 mm, showed a significant difference (p<0.05) compared to the Y treatment 1.35 ± 0.01 mm and 1.11 ± 0.01 mm Therefore, this study concluded that tryptophan supplementation of 0.49% in dry feed had a positive effect on accelerating the maturation of catfish starting at 20 days of treatment with the following hormone levels, melatonin of 10.69±0.14 ng mL-1, estradiol 1.90 ± 0.03 ng mL-1, vitellogenin 35.84 ± 0.37 ng mL-1, and oocyte diameter of 1.05 ± 0.09 mm.
Keywords | Reproduction, Estradiol, Vitellogenin, Oocyte, Egg diameters, Feed
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