Association of c.974C>G Mutation in Growth Differentiation Factor 9 with Recovery Rate in Peranakan Ongole Cattle
Association of c.974C>G Mutation in Growth Differentiation Factor 9 with Recovery Rate in Peranakan Ongole Cattle
Irma1*, Siti Darodjah Rasad2, Nena Hilmia2, Cece Sumantri3
ABSTRACT
Growth Differentiation Factor 9 (GDF9) is related to female reproductive characteristics, particularly folliculogenesis. The significant effect of GDF9 has been explored in previous investigations by determining the association between genetic diversity and phenotypic variations using c.589T>G, c.659T>G, c.974C>G, c.1105T>A, and c.1358G>A from Polymerase Chain Reaction (PCR) based on direct sequencing. This study aimed to analyze the association between GDF9 diversity and the superovulation response in Peranakan Ongole (PO), Belgian Blue (BB), and its crossbreeds (BB x PO). The experiment was conducted using 21 donors from Livestock Embryo Centre (LEC), which were selected for the superovulation based on oestrous synchronization. The normality distribution of data was evaluated using Kolmogorov-Smirnov, followed by non-parametric analysis. Furthermore, Single Nucleotide Polymorphism (SNP) was used to determine genotypes and their association was analyzed with the Kruskal-Wallis statistical test. In PO, SNP c.974C>G on GDF9 showed that GG genotypes had a higher recovery rate (82.83±5.83%) compared to CC genotypes (69.27±6.62%), with p<0.05. Based on c.589T>G, c.659T>G, c.974C>G, c.1105T>A, and c.1358G>A, mutant haplotype had a higher recovery rate (86.06±6.06%) compared to wild haplotype (69.06±6.14%), with p<0.05. The result showed that SNP at c.974C>G could be used as a marker for recovery rate in assisted selection and livestock breeding programs in the breeding centre location.
Keywords | GDF9, Mutation, Cattle, Recovery rate, Superovulation
To share on other social networks, click on any share button. What are these?
