Effects of Dietary Tryptophan Supplementation on Melatonin and Oocyte Maturation in African Catfish, Clarias Gariepinus
Epro Barades1,4*, Iskandar2, Ibnu Dwi Buwono2, Yuli Andriani2, Ayi Yustiati2, Roffi Grandiosa2, Ratu Siti Aliah3
1Agricultural Science Study Program, Faculty of Agriculture, Universitas Padjadjaran, Sumedang, West Java, Indonesia; 2Department of Fisheries, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Sumedang, West Java, Indonesia; 3Research Center for Fishery, National Research and Innovation Agency, Indonesia; 4Fish Hatchery Technology Study Program, Politeknik Negeri Lampung, Bandar Lampung, Indonesia.
*Correspondence | Epro Barades, Agricultural Science Study Program, Faculty of Agriculture, Universitas Padjadjaran, Sumedang, West Java, Indonesia; Email:
[email protected]
Figure 1:
Trp and endocrine regulatory scheme on oocyte growth and maturation in fish (adapted from Babin et al., 2007; Finn and Cerdà, 2024; Pavlov and Emel’yanova, 2016). Abbreviations: GnRH, gonadotropin-releasing hormone; LH, Luteinizing hormone; FSH, follicle-stimulating hormone.
Figure 2:
Mel Levels in catfish broodstock after treatment with Trp. Error bars represent the standard deviation (SD) of the mean. The superscript letters above the error bar and observation time show a significant difference between the means using the DMRT multiple range test at p< 0.05. Dashed lines separate the DMRT test results for observation times. If there is no significant difference due to the observation time, the difference in hormone levels at that time will use superscript letters above the error bars with the same letter range.
Figure 3:
E2 Levels in catfish broodstock after treatment with Trp. Error bars represent the standard deviation (SD) of the mean. The superscript letters above the error bar and observation time show a significant difference between the means using the DMRT multiple range test at p< 0.05. Dashed lines separate the DMRT test results for observation times. If there is no significant difference due to the observation time, the difference in hormone levels at that time will use superscript letters above the error bars with the same letter range.
Figure 4:
VTG Levels in catfish broodstock after treatment with Trp. Error bars represent the standard deviation (SD) of the mean. The superscript letters above the error bar and observation time show a significant difference between the means using the DMRT multiple range test at p< 0.05. Dashed lines separate the DMRT test results for observation times. If there is no significant difference due to the observation time, the difference in hormone levels at that time will use superscript letters above the error bars with the same letter range.
Figure 5:
Gonadal histology after 30 days of treatment. Germinal vesicle, gv; yolk platelet, yp; yolk cavities, yc; oil, o; chorion, c. Bar scale = 1 mm, magnification 40×.