Polymorphism in the Exon 11 of Bovine Luteinizing Hormone Receptor Gene and its Association with Service per Conception in Pasundan Cows
Polymorphism in the Exon 11 of Bovine Luteinizing Hormone Receptor Gene and its Association with Service per Conception in Pasundan Cows
Syahruddin Said1*, Widya Pintaka Bayu Putra1 and Dani Nur Arifin2
ABSTRACT
Bovine luteinizing hormone receptor (bLHR) gene in mammals is important for several reproduction function such as steroidogenesis, follicular growth, oocytes maturation, ovulation and corpus luteum formation. The aim of this study was to identify the polymorphisms in the exon 11 of bLHR/HhaI gene (SNP g.1553C>T) and its association with service per conception (S/C) of Pasundan cows of West Java, Indonesia. Total of 147 Pasundan cows were used in this study. The PCR-RFLP analysed three genotypes of CC (0.11), CT (0.57) and TT (0.32) were detected in the Pasundan bLHR/HhaI gene with T allele as the dominant allele (0.61). Observed (Ho) and expected (He) heterozygosity had similar values of 0.48. The polymorphic informative content (PIC) was 0.37 and was of moderate category (0.25<PIC<0.50). The Chi-square value (χ2) was 5.71, which reveals that studied population is under the Hardy-Weinberg equilibrium (χ2<5.991). The number of effective allele (ne) was 1.92. It showed that Pasundan bLHR/HhaI had two common alleles. Based on the sequencing analysis results of 46 samples, it showed that new mutation at g.1626G>T was only detected in one individual sample. Polymorphism of bLHR/HhaI gene in the present study was not significantly associated with S/C of Pasundan cows, since the genotype TT of the studied animal was found in the lowest of S/C value than other genotype.
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June 2021
Vol. 53, Iss. 3, Pages 801-1200
