B. Ramanujam, R. D. Prasad, S. Sriram and R. Rangeswaran
Mass production, formulation, quality control and delivery of Trichoderma for plant disease management
...n inexpensive media like molasses and yeast medium in deep tanks on a commercial scale. Biomass from the liquid fermentation can be made into different formulations like, dusts, granules, pellets, wettable powders. Trichoderma formulations can be applied to the seed either by dry seed treatment or by seed biopriming for control of several soil-borne diseases of some field crops. Similarly, seedlings of horticultural crops and rice are treated by dipping the ro...
Nasia Batool*, Imtiaz Ahmad Qamar**, Imdad Hussain Mirza** and Muhammad Fateh Ullah Khan**
MIXING LESS PALATABLE GRASSES WITH UREA, MOLASSES AND EFFECTIVE MICROORGANISMS AND ITS EFFECT ON CHEMICAL COMPOSITION AND DIGESTIBILITY IN GOATS
...table grasses with urea, molasses and Effective Microorganisms (EM) on chemical composition and digestibility in goats. (HC), (CA), (SH) and (DB) were used and the combinations were grass + 4% molasses, grass + 4% urea, grass + 4% urea + 4% molasses, grass + 4% urea + 1:100 EM, grass + 1:100 EM + 4% molasses, grass +1:100 EM + 4%
*Irum Javed1, Muhammad Asgher2, Sadia Noreen1, Humara Naz Majeed1 and Tanzila Sahar1
SOLID STATE FERMENTATION OF ASPERGILLUS NIGER FOR CITRIC ACID PRODUCTION USING AGRICULTURE RESIDUE AS SUBSTRATE
...niger was compared using molasses based medium and the different carrier substrate. In a second set of study wheat bran was utilized for citric acid production via fermentation parameters was optimized. The results showed significant (P≤ 0.05) difference among LSF and SSF and between different substrates in LSF and SSF. Wheat bran showed significantly increased (P≤ 0.05) production of citric acid (P= 0.001) compared to other carrier substrates used throu...
Unal Kilic1*, Abdiwali Mohamoud Abdi1 and Deniz Ekinci2
...s (control, 4% urea, 10% molasses and 14% urea+molasses (4% urea, 10% molasses). Hohenheim gas test was used in in vitro gas production measurements while infrared methane analyzer was used to determine methane production. The experiments were conducted as per the randomized factorial design in randomized complete blocks. In terms of crude protein content, the highest CP was obtaine...
Muhammad Irfan*, Saeed Gul*, Mohammad Younas*, Babar Nawaz*, M. Ishfaq*, M. Amir Durrani*, Abid khan*
FERMENTATION OF SUGARCANE MOLASSES BY SACCHARAMYCES CEREVESIA: EFFECTS OF OPERATING PARAMETERS ON ETHANOL PRODUCTION
...for massive production ofmolasses. Typically the molasses is converted to ethanol through fermentation process. A pilot plant has been designed and fabricated to investigate the effect ofprocess parameters for the su...
Tehmina Aslam, Shagufta Saeed*, Muhammed Tayyab, Huma Mujahid, Ali Raza Awan, Sehrish Firyal and AbuSaeed Hashmi
...bran, rice polishing and molasses for the synthesis of polyhydroxybutyrate (PHB) by Azotobacter vinelandii. The optimization of different carbon sources and various physical parameters was also performed. Highest yield of PHB was obtained by fermentation of 4% (w/v) wheat bran after 48 h of incubation with 4% (v/v) inoculum volume at pH 7.0 and 37oC. Among different nitrogen sources tested, 0.2% peptone gave the better yield (285 mg/100 mL), ...
Faheem Ahmed Khan1*, Sarzamin Khan2, Qurat-ul-Ain3, Saqib Ishaq1, Muhammad Salman1, Abdul Rehman1, Ikram Ullah4, Kalsoom5 and Johar Jamil5
...as produced on sugarcane molasses (6.42 g/l) followed by orange peels. Microbial biomass produced on the sugarcane molasses was rich in crude proteins (56 %) followed by that from orange peels. Highest percentage of alanine (24.72%) and aspartic acid (17.83% and 14.57%) were observed in dried single cell microbial protein produced on sugarcane molasses, orange peels and apple waste, respec...
Amjed Ali1, Muhammad Tayyab1,*, Sehrish Firyal1, Abu Saeed Hashmi2, Asif Nadeem3, Shagufta Saeed1, Ali Raza Awan1 and Muhammad Wasim1
...heat bran, rice bran and molasses could increase the enzyme production from 25 to 43.7, 44.3 and 49 µmole/min whereas the nitrogen sources including tryptone, peptone and yeast extract could enhance the enzyme production from 25 to 40.21, 41 and 43.76 µmole/min, respectively. The highest β-lactamase production was recorded when the LB medium was supplemented with 4% wheat bran and 2% yeast extract. The locally produced recombinant β-lacta...
Muhammad Mohsin Javed1*, Muhammad Azhar Nisar2 and
Muhammad Usman Ahmad2
...ctricity production from molasses has not been studied considerably. Present study describes the use of molasses with different organic loads in MFC-1, MFC-2 and MFC-3 with 10, 15 and 20% of sugar contents, respectively, for bioelectricity production. These MFCs were designed locally and maximum power densities of 72820mW/m2, 104400mW/m2and 44340mW/m2for MFC-1, MFC-2 and MFC-3, respectively w...
Najeeb Ullah1*, Muhammad Nadeem2, Malik Mujaddad-ur-Rehman1, Rubina Nelofer2 and Yasir Arfat3
...ty was observed by using molasses (540.62 ± 26.77 IU/ml) as carbon source and NaNO3 (606.95 ± 7.61 IU/ml) as nitrogen source. The optimum conditions predicted by RSM were substrate 2%, molasses 1.5%, temperature 35 °C, pH 9.0 and induction period of 96 hour. Almost 2.9 fold increase in protease production was observed after optimization at these optimal conditions. All these results indicated that skin wast...
K.M. Injarul Haque, Sharmeen Islam*, Md. Rokibul Islam Khan, Md. Ruhul Amin
...rumen contents (RC), and molasses to determine the physical and nutritional properties of silages. Silages were prepared in airtight plastic containers at ambient temperature according to the treatments T0 (0% RC), T1 (5% RC), T2 (10% RC), and T3 (15% RC) with 5% molasses as dry matter basis at 0, 14, 21, and 28 days, respectively. Results showed that the physical qualities (color, odor, and softness) of silages were improve...
Preparation and Evaluation of Cattle Feed by Ensiling Wet Rice Straw, Market Fish Waste and Molasses
Avijite Sarker, Sharmeen Islam, S.M. Ariful Islam, Md. Rokibul Islam Khan*, Md Mukhlesur Rahman
...W) and fermented with 5% molasses to enhance the storage quality and nutrients of rice straw. Feeds were placed in plastic drums and sealed properly. The preparation was completed under room temperature following T0 (0% MFW), T1 (5% MFW), T2 (10% MFW), and T3 (15% MFW) to examine the physical qualities, metabolizable Energy (ME), in vitro organic matter digestibility (OMD) and other nutritional properties, at different intervals (0, 30, 45, and 60 days). The o...
Nguyen Hai Quan, Nguyen Huu Van*, Nguyen Thanh Thuy, Vo Thi Minh Tam, Le Duc Thao, Le Duc Ngoan
Desy Cahya Widianingrum1*, Himmatul Khasanah1, Melinda Erdya Krismaputri1, Susan Barbara Patricia Sembiring Meliala2
...rate, 30% indigofera, 5% molasses, and 5% VCO. The group of treatments were wafer supplemented with VCO (P0); wafer supplemented with VCO saponification (P1); wafer supplemented with VCO protected by formaldehyde (P2); wafer without VCO (P3); and wafer supplemented with VCO mixed on indigofera (P4). Each treatments were using six replications and tested for their physical quality, rancidity, and rumen fermentation characteristics and in vitro digestibility. Th...
Faustin Dokui1*, Frédéric M. Houndonougbo1, Service G. Djidda1, Venant P. Houndonougbo1, Edith Gangbedji1, Gwladys Menon Agbo2, Sedjro Ludolphe Dedome2, Séverin Babatoundé1, Soumanou Seibou Toleba1, Christophe A.A.M. Chrysostome1
...ich in crude protein and molasses, was higher compared to the other groups during the dry season and higher than the intake of the treatment group fed with salt-poor stone during the experiment period. Cows in the LS2 treatment group produced much more milk during the dry season than the other treatment groups. Cows in the LS3 treatment group fed with stone low in salt produced much more milk from the fifth week to the end of the trial than the other treatment...
SAIMA AFTAB1, ATTIA HAMID1, MAHJBEEN2, JAVERIA TARIQ2, ZULQURNAIN ALI2, ASMA ZAFAR2*
...arch-based substrate and molasses, and other wastes. In this study low-quality apples were used for the production of ethanol that was further used for various purposes. From this work, it is clear that the maximum ethanol production is obtained from the apples. S. cerevisiae strain was used for the maximum conversion of sugar contents of apple extracts into ethanol. Different parameters like incubation time, temperature, pH and Inoculum size were optim...
SAIMA AFTAB1, ATTIA HAMID1, MAHJBEEN2, JAVERIA TARIQ2, ZULQURNAIN ALI2, ASMA ZAFAR2*
...arch-based substrate and molasses, and other wastes. In this study low-quality apples were used for the production of ethanol that was further used for various purposes. From this work, it is clear that the maximum ethanol production is obtained from the apples. S. cerevisiae strain was used for the maximum conversion of sugar contents of apple extracts into ethanol. Different parameters like incubation time, temperature, pH and Inoculum size were optim...
Ovirup Bhushan Paul1, Shodipta Sharma Urmi2, Md. Ashraf Ali Biswas3*
... by mixing TMR feed with molasses containing Saccharomyces cerevisiae. Prepared TMR and FTMR feed mixed with rumen fluid buffer for in vitro digestion at 6h, 12h, 24h, & 48h of the incubation period. There were five replications at each of TMR and FTMR dietary treatments. Mean comparison of both TMR and FTMR group evaluated by t-test assuming equal variances. We observed decreasing tendency of pH with the increasing incubation period and no significant di...
Khalil1*, Dwi Ananta1, Andri Bachtiar2, Hermon3
...ed mineral mixture, 0.6% molasses, and 0.05% urea. The supplemented straws were stored for 60 days in three bulk treatments: loose stacking, open-tied rolling, and airtight wrapping. The changes in physical appearances, botanical fraction, proximate, and fiber fraction content were determined. The nutritive values of stored rice straws were evaluated by feeding four male Indonesian-indigenous Pesisir cattle in a 4x4 Latin Square design. There were four experim...
Limbang Kustiawan Nuswantara, Eko Pangestu, Marry Christiyanto, Santoso Dwi Pratomo, Elyza Zahrotul Muhtaromah
...oybean meal, wheat bran, molasses, gamal leaf flour, calliandra leaf flour. and indigofera leaf flour and then made in pellets. The experimental design used was a randomized block design (RBD) based on grouping body weights consisting of 4 groups and 4 treatments. The treatment applied was a balance of feed in the form of forage elephant grass with different levels concentrat of 25, 50 and 75%, and control treatment in the form of rations that are usually give...
A. W. Jasra and I. Ahmad
...rigation system and urea molasses blocks as supplemental feed have been identified as viable interventions for livelihood improvement. A policy dialogue is needed to resolve the problems of nomadic community.
Key Words: Pastoralists, livelihood, Juniper Forest, Balochistan, Pakistan
...
Budi Santoso*, Bambang Tjahyono Hariadi, Marlyn Nelce Lekitoo
...(10%), tofu waste (10%), molasses (7%), lactic acid bacteria (LAB) inoculant (3%), and cellulase 0 ml/kg. Treatment B comprised silage A + 3 ml cellulase/kg, while C used silage A + 6 ml cellulase/kg. Furthermore, treatment D consisted of palm frond (50%), king grass (20%), cassava (10%), tofu waste (10%), molasses (7%), LAB inoculant (3%), and cellulase 0 ml/kg. Treatment E used silage D + 3 ml cellulase/kg, while F was com...
Ahsan Abbas Abro1, Imrana Khushk1, Abdul Nabi Jatt2, Chaudhary Haider Ali3, Abdul Sattar Qureshi1*, Farah Naz Talpur4, Safia Bano1
...el. Fungus cultivated in molasses mineral medium with tryptone as the nitrogen source for 5 days at 37 °C and 6.0 pH, strain produced a maximum lipase concentration of 29.39 (U/mL). Effect of metal ions, pH, pH stability, temperature, and thermostability on lipase activity were examined. Transformation of used oil into biodiesel was carried out by employing lipase produced in the current work. Maximal fatty acid methyl ester formation was noted after 18 h,...
Muhammad Ambar Islahuddin1, Yuli Retnani2*, Despal2
...the effects of different molasses viscosities and various binder options on the physicochemical quality of wafer supplements containing prill fat, as well as their impact on fermentation and digestibility in dairy cows in vitro. Molasses with viscosities of 320 mPas-1 and 6400 mPas-1 were utilized alongside pollard binders, cassava meal, and cassava waste. The experimental design employed a 2x3 factorial pattern CRD with 5 r...
A. Arif Rahman1, Renny Fatmyah Utamy2*, Ambo Ako2, Mohammad Mijanur Rahman3, Siti Annisa Sukri1
...">The production of urea molasses multinutrient block (UMMB) typically involves cement and molasses. However, prolonged use of cement can lead to health issues in livestock, while molasses is often in short supply due to its extensive use as a raw material in various industries. Thus, alternative materials are highly needed as substitutes for both cement and molass...
Abd-Elhalim, B.T.1*; Gamal, R.F.1; Abou-Taleb, Kh.A.1; Haroun, A.A.2
Novel Research in Microbiology Journal (2019), 3(6): 558-578
...2% blackstrap sugar cane molasses was the most efficient carbon source for CuNPs biosynthesis, when incubated for at 30°C for 24 h using shaking speed of 120 rpm. The biosynthesized CuNPs has a size of 66.12 nm at a concentration of 19.2 mg\ l, and maximum surface plasmon peak (SPR) of 0.85....
Dhurva Prasad Gauchan1*; Ashok Kumar Bhattarai2; Shishir Pandey1; Sunil Bhandari1
Novel Research in Microbiology Journal (2020), 4(4): 921-938
...lk, rice husk, jackfruit molasses and sugarcane bagasse) through solid state fermentation (SSF). Production of conidia (cfu/ g) was higher in corn stalk (72.6 ×108), followed by rice husk (68.4×108), jackfruit molasses (18.6×108) and sugarcane bagasse (12.4×108). High counts of conidia production on these substrates render MUSH isolate efficient to be used as a BCA by the farmers, to enhance their cro...
R.F. Utamy1*, A. Ako1, H. Hasbi1, Z. Ramadan2, R. Mufliha2, A. Mutfaidah2, A. F. Nurbina2, I. D. A. Mahayani3, A. A. R. Hakim2,4
...xt-align: justify;">Urea molasses multi-nutrient block (UMMB) supplementation is a method employed to enhance the performance of Holstein Friesian dairy cows. However, many UMMBs use cement adhesive as a key component. Some concerns are that using cement as an adhesive could adversely affect the health of dairy cows if consumed over an extended period. Therefore, it is necessary to find organic materials to replace cement as an adhesive component for UMMBs. On...
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