Effect of TMR and Fermented TMR on Ruminal In vitro Digestion and Gas Production
Effect of TMR and Fermented TMR on Ruminal In vitro Digestion and Gas Production
Ovirup Bhushan Paul1, Shodipta Sharma Urmi2, Md. Ashraf Ali Biswas3*
ABSTRACT
Ruminants efficiently digest fibrous components resulting in high-quality milk and meat. The emerging ruminant population to meet the protein demand is one of the driving factors of increasing global greenhouse gases. The current study aimed to assess the effects of total mixed ration (TMR) and fermented TMR (FTMR) feed on digestibility, total gas production and pH of ruminants. Novel feeding techniques were implemented that combined fermentation to improve digestibility with locally accessible feed supplies using a completely randomized design. TMR feed was produced with 70% roughage of silage & rice straw and 30% concentrate mixture. FTMR feed was produced by mixing TMR feed with molasses containing Saccharomyces cerevisiae. Prepared TMR and FTMR feed mixed with rumen fluid buffer for in vitro digestion at 6h, 12h, 24h, & 48h of the incubation period. There were five replications at each of TMR and FTMR dietary treatments. Mean comparison of both TMR and FTMR group evaluated by t-test assuming equal variances. We observed decreasing tendency of pH with the increasing incubation period and no significant difference of pH between TMR & FTMR feeds at 24h, 48h incubation period. The highest mean digestibility of both TMR and FTMR feed was obtained at 34.78% (34.78±1.28) and 45.91% (45.91±1.09) respectively, at 48h incubation period. Among five replicates of both TMR and FTMR, the highest mean gas production was 74ml (73.8±1.11) and 59ml (59±0.89) respectively, at a 48h incubation period. The digestibility of FTMR was significantly higher than the TMR (p<0.01) feed at all incubation periods. Conversely, total gas production was significantly lower in FTMR than in the TMR (p<0.01) feed. It can be concluded that FTMR is better than TMR in terms of digestibility and gas production.
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