Unveiling the Safety of Cassia alata Leaf Extract: A Subchronic Oral Toxicity in Rats
Unveiling the Safety of Cassia alata Leaf Extract: A Subchronic Oral Toxicity in Rats
Amaq Fadholly1,5*, Siti Sa’diah1,6, Diah Nugrahani Pristihadi1, Eva Harlina2, Maharani Kartika Ramadhan3, Yumna Annisa Kurnia Budiharjo4, Cyntia Rafi Adinugroho4, Afifah Alawiyah Rahmah4, Nahla Fifi Azizah4
ABSTRACT
Cassia alata has been employed as a traditional herbal medicine for several disorders, demonstrating anticancer, antiallergic, antioxidant, anti-inflammatory, antifungal, and antidiabetic properties. The main aim of this study was to evaluate the subchronic oral toxicity of the extract obtained from Cassia alata leaves (CALE). Male and female rats were orally administered four different dosages of CALE (0, 100, 300, and 900 mg/kg/day) for a duration of 28 days. Hematological and biochemical data, organ weights, histological findings, clinical symptoms, mortality rates, food and fluid intake, and fluctuations in body weight were rigorously observed. The CALE data reveal no statistically significant changes in mortality is 0%, hematological, biochemical and organ weights parameters are within normal range, food and water consumption is normal, body weight increases with age and no clinical symptoms appear. The histopathological analysis indicated no changes in the kidneys or liver. We conclude that no observed adverse effects of rats for both sexes after 28 days of oral CALE treatment at ≤ 900 mg/kg/day. CALE has the potential to develop into herbal medicine.
Keywords | Cassia alata, Herb medicine, OECD guidelines, Phytochemical analysis, Safety assessment, Subchronic toxicity
To share on other social networks, click on any share button. What are these?
