This study aimed to evaluate the effects of a combined PCB (phytobiotic, chromium, and betaine) supplementation in the diet on the performance, egg quality, and health status of ISA Brown laying hens. A total of 2000 ISA Brown laying hens were used, divided into four treatment groups with five replicates each. The treatments were: T1 = control diet without supplementation, T2 = control diet supplemented with 0.05% PCB, T3 = 0.10% PCB, and T4 = 0.15% PCB. Data were analyzed using analysis of variance (ANOVA) in SPSS Statistics, and significant results were further examined with Duncan’s multiple range test. The results showed that PCB supplementation at 0.05% and 0.10% significantly (P<0.05) increased hen-day production, egg mass, eggshell color, and blood eosinophil levels, while also decreased (P<0.05) daily feed intake, feed conversion ratio, and egg weight. In conclusion, 0.05% and 0.10% PCB increased egg production by 13% and improved feed efficiency by 9% in 82–87 week laying hens. Suplementation of PCB is an effective strategy for improving laying hen productivity and feed efeciency.
Keywords | Egg quality, Supplementation, Immunity, ISA Brown hens, Performance