Search for Polysaccharides

Evaluation of the Ability of Anti-Lipopolysaccharide Aptamers to Discriminate Various Pathogenic E. coli Bacteria

John G. Bruno

...ed against extracted lipopolysaccharides (LPS) of E. coli O157 and the so called “Big six” non-O157 Shiga-toxin producing E. coli (STEC) serovars (O26, O45, O103, O111, O121 and O145). These aptamers were assessed by enzyme-linked aptamer sorbent assay (ELASA) to rank their relative affinities and discriminatory ability for distinguishing between each of the STEC serovars. The most specific biotinylated aptamers from the ELASA screening were then a...

Characterization of Staphylococcus aureus from Sore Throat Patients: Association Among Host Immune Evasion and Toxin Genes

Arifa Mehreen¹, Iram Liaqat²,Muhammad Arshad³, Muzzamil Waheed⁴ and Najma Arshad^1,*

... proportions of capsular polysaccharides (CPs8), clumping factor A (clf A) andintracellular adhesion A (ica A) were 78%, 68.5% and 40%, respectively. ica D and CPs5 could not be amplified from any isolate. Toxin genes were present in 43.5% isolates. Among toxin genes, enterotoxins (SEs) were most frequently detected followed by enterotoxin (ETs) (24.09 %) and toxic shock syndrome toxin (TSST-1) (15...

Screening and Identification of Chitin Degrading Bacteria from Local Environment

Farah Mansha, Abdul Rehman*

... used to degrade complex polysaccharides present in the organic wastes and would be helpful in cleaning the environment.

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Encapsulation of Pumpkin Seed Oil in Alginate Capsules

Asma Sohail^1*, Kashif Sarfraz Abbasi¹, Maryum Arif² and Fatima Najam¹

...ve ingredients including polysaccharides, vitamins, proteins, essential amino acids, carotenoids, fatty acids like linoleic acid and linolenic acid, lignans, minerals and various phytochemicals. Due to the presence of two unsaturated fatty acids, these oils can reduce their biological activity on heating, volatilization, and other parameters like oxidation and UV rays. This limits the commercial application of these oils. To overcome such problems encapsulatio...

Antibacterial Activity of Capsaicin against Sectional Cariogenic Bacteria

Hao Gu*, Zhi Yang, Weiqiang Yu, Kan Xu and Yuan-fei Fu*

...fluorescent images of exopolysaccharides and living bacteria of Streptococcus mutans were measured with confocal laser scanning microscopy (CLSM). In our study, capsaicin showed an outstanding performance in the inhibition of acid producing and biofilm of the four strains of cariogenic bacteria. Meanwhile, low values of MIC of capsaicin on four sectional cariogenic bacteria indicated high efficiency of antibacterial activity of capsaicin. Antibacterial ...

Characterization of Thermophilic Bacteria Anoxybacillus rupiensis and Cultivation in Agroindustrial Wastes Isolated from Hot Spring in Chakwal, Pakistan

Faiza Jabeen^1,*, Bushra Muneer² and Javed Iqbal Qazi³

... mannitol, Arabinose and polysaccharides like gelatin and starch. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both isolates belong to the same specie Anoxybacillus rupiensis with 99% similarity under Accession number KF254911 (JF82) and KC849452 (JF83). The DNA G + C contents were 56.854% and 56.754% for A. rupiensis JF82 and A. rupiensis JF83, respectively. The isolates grew over a wide range of temperatures (40-7...

Recovery of Chemotherapy-induced Anemia by Angelica Polysaccharides in C57BL/6N Mice and Elucidation of Underlying Mechanism

Tian-Yi Zhao¹, Zi-Qing Liu², Bo Yang¹, Qiao Gao¹, Hong-Yu Zhang¹, Pei-Yu He¹, Ming-Hua Duan¹* and Yu-Li Yan¹*

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Analysis of Cell Surface Properties of Exiguobacterium aurentiacum Associated with Lumbricus terrestris and its Potency as Biological Inoculants

Syeda Shazia Bokhari, Aisha Waheed Qurashi*, Roheela Yasmeen*, Fouzia Yasmeen, Nabeela Nayab, Uzma Rafi

...towards adherence and exopolysaccharides productions. Both productions are important parameters of plant growth promoting bacteria. Earthworms, along with soil sample, were collected from different areas of Lahore. Bacteria were isolated, purified and further characterized morphologically, biochemically and at molecular level. Bacteria after isolation were labelled as EPF1 while by molecular confirmation this bacterial strain was named as Exigoubacterium auran...

Histomorphology and Histochemistry of the Oviduct in Laying Turkey Hens with Emphasis on the Sperm Host Glands

Taghreed Mohamed Nabil1, Randa Mohamed Hassan1, HebatAllah Hamdy Mahmoud2, Mohamed Gomaa Tawfiek2, Usama Kamal Moawad1*

... glands. The neutral mucopolysaccharides were predominant in supra-nuclear portions of the mucosal epithelium in all portions of the oviduct. In contrast, acid mucopolysaccharides were dominated in the infundibulum, magnum, isthmus, and vagina but were absent in the proprial glands. Sperm host glands were prominent in the uterovaginal portion. The vaginal tunica muscularis was the thickest compared with other oviduct parts f...

Effects of Dietary Microalgae Supplementation on Mammals’ Production and Health

Aiman Al-Mufarji¹, Abd El-Nasser Ahmed Mohammed¹*, Haitham Al-Masruri², Rashid Al-Zeidi³

...include “proteins, polysaccharides, lipids, PUFAs, vitamins, pigments and other bioactive compounds”. The microalgae composition varies depending on species and nutrient availability for production. Genetic engineering might be used for production invaluable microalgae compounds. Microalgae consider a promising source of omega-3 fatty acids (n-3 FAs), which have beneficial effects for humans and animals. Knowledge for microalgae effects on producti...

The Effect of Lactobacillus acidophilus on Alleviating Stress Response and Production Impairment Induced by Escherichia coli Lipopolysaccharide in Laying Hens

Abdulaziz A. Alaqil*

...enously injection of lipopolysaccharides (LPS). A total of one hundred twenty, 40-week-old, laying hens from the Hy-line breed were allocated at random into four groups of 30 hens each. For eight consecutive weeks (40–48 weeks of age), two groups of the layers were given a basal diet as control, while the remaining two groups were given a basal diet enhanced with a 2 g/kg diet of LA bacteria. At 47 wk of age, layers in one of the control or LA group...

Glucomannan Potential of Banana Corms (Musa paradisiaca) as Prebiotics

Nurul Fajrih^1,2, Komang Gede Wiryawan¹*, Sumiati¹ and Suraya Kaffi Syahpura³

Bacterial Exopolysaccharides: sources, production and applications

MARIAM ZAHEER*1, SYED SHAHID IMRAN BUKHARI2, MUDDA

...text-align: justify;">Exopolysaccharides (EPSs) are secreted by both eukaryotes (fungi, phytoplankton, and algae) and prokaryotes (archaeobacteria and eubacteria). EPSs produced by bacteria include a broad range of chemical structures and may either be heteropolymeric or homopolymeric. Their molecular weights vary from 10 to 1000 kDa. Exopolysaccharides have multifarious applications in different fields of life. These EPSs a...

Pharmacological activities of Oyster mushroom (Pleurotus ostreatus)

Girma Waktola1; Tasisa Temesgen2*

Novel Research in Microbiology Journal (2020), 4(2): 688-695

...ot;;">mushroom comprise: polysaccharides, lipopolysaccharides, proteins, peptides, glycoproteins, nucleosides, tri- terpenoids, lectins, lipids and their derivatives, in addition of its vital medicinal components beneficial for the human health. Moreover, Pleurotus ostreatus

Evaluation of chitinolytic enzymes activities during microbial biotransformation of Archachatina marginata exoskeleton to chitooligosaccharide

Simiat O. Jimoh1*; Khadijat O. Adefaye1; Lateefah A. Arowolo2; Ramot B. Badmos-Oladapo1

Novel Research in Microbiology Journal (2022), 6(2): 1530-1542

...l macromolecules such as polysaccharides are bioactive substances obtained from the agricultural feed stocks and\ or crustacean shell wastes, thus chitin biomass as a rich renewable resource; is an additional abundant natural polysaccharide in the environment. Despite the recent interests in the biomedical applications of chitin, complexity of chitosan and their derivatives in terms of controlling their chemical synthesis due to the formation of secondary comp...

Uropathogenic Escherichia coli in urinary tract infections: A review on epidemiology, pathogenesis, clinical manifestation, diagnosis, treatments and prevention

Bijay Kumar Shrestha1*; Manita Tumbahangphe1; Jenish Shakya1; Sujata Chauhan1

Novel Research in Microbiology Journal (2022), 6(4): 1614-1634

...aemolysins, capsule, lipopolysaccharides, biofilm, fimbriae adhesions and iron acquisition systems. In spite of several host protection mechanisms; however, UPEC may persist inside the urinary tract and serve as a reservoir of recurrent infections and complications. Early diagnosis and prompt treatment of UTI with broad spectrum antibiotics are essential before this infection causes other medical complications. Generally, in clinical settings, diagnosis of UTI...

Probiotics as promoters of human health

Mai N. Amer1; Maissara M.K. Elmaghraby2; Aya A.M. Abdellatif2; Sondos R. Salem3; Mariam M. Salama3; Omar A. Abdel Maksoud3; Radwa M. Nasr3; Mohamed N.H. Amer4; Mohamed M.H. Marghany5; Hassan M. Awad1*

Novel Research in Microbiology Journal (2024), 8(5): 2580-2603

...nzymes, vitamins, and exopolysaccharides with antibacterial attributes. Consequently, probiotics are utilized in food technology to develop the functional foods that promote the human health.

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Comprehensive insights into colistin and colistin resistance: updated regulations and policies on colistin usage and alternative strategies for mitigation and prevention of colistin resistance

Zine El abidine Bzazou El Ouazzani1*; Laila Reklaoui1; Monsif El Madany2; Houda Benaicha3; Said Barrijal1

Novel Research in Microbiology Journal (2024), 8(5): 2632-2652

...eracting with
lipopolysaccharides (LPS), causing cell death. Currently, colistin is regarded as a last-resort
antibiotic for treating infections caused by Gram-negative bacteria (GNB). Overuse of colistin
in animal feed and human medicine has led to the appearance of genetic mutations and the
acquisition of mobile colistin resistance (mcr) genes associated with colistin resistance. The
rapid emergence of the plasmid colistin-...

Aquaporin-9 Aggravates Lipopolysaccharides Induced Acute Lung Injury Via Facilitating M1-Like Macrophage Polarization

Jiajia Wan^1,2, Chi Zhang³, Xiaodi Li³, Longkang Liu³, Linxiu Peng^3,4, Yuejian Liu², Yang Qiu², Guokai Wu², Bo Zhang³*, Yan Wang³* and Tonghui Ma³*

...regulation following lipopolysaccharides (LPS) administration. AQP9 gene knockout (AQP9 KO) mice exhibited markedly attenuated alveolar hemorrhage and interstitial edema in an LPS-induced ALI model compared with wild-type (WT) littermates. The mitigated lung injury in AQP9 KO mice correlated with significantly alleviated pulmonary inflammation as indicated by significantly reduced immunofluorescent staining of CD68+ macrophages in the lungs, decreased level of...