Genotoxicity and Anti-Cancer Activity of Tamoxifen and Ivermectin Loaded Chitosan Nanoparticles Against MCF-7 Cell Line
Genotoxicity and Anti-Cancer Activity of Tamoxifen and Ivermectin Loaded Chitosan Nanoparticles Against MCF-7 Cell Line
Usama Bin Naeem1, Muhammad Adil Rasheed1*, Muhammad Ashraf1 and Muhammad Yasir Zahoor2
ABSTRACT
The purpose of this work was to examine the anti-proliferative activity of tamoxifen (TAM) and ivermectin (IVM) loaded chitosan (Cs) nanoparticles against breast cancer cell line (MCF-7) to improve drug delivery to the target sites. Characterization of NPs were carried out by Fourier transform infrared spectroscopy, zeta sizer, potential, morphology by scanning electron microscope, drug entrapment efficiency and in vitro drug release. In TAM and IVM loaded chitosan nanoparticles the peaks were at 1606 cm-1 and 1640 cm-1 respectively. TAM and IVM-CsNPs have size 189 and 196 nm with positive charge. The morphology of both nanoparticles were spherical and smooth surface. Drug entrapment efficiency was 79.08 ± 1.3 % and 89.00 ± 0.043 % respectively. At pH 6.0 and 7.4, drug released from Cs-NPs in biphasic manner, with an initial burst occurring during first 6–12 h and a steady release lasting for 48 h. Inhibitory concentration value of free drugs and nanoparticles alone and in combination with selectivity index (SI) were calculated that was > 2. SI of drugs and CsNPs are > 2 which showed cytotoxicity towards cancerous cells. Genotoxicity assessment showed slight damage by TAM and IVM to the mean tail length of DNA as compared to nanoparticles. These results suggest that TAM and IVM loaded CsNPs may be promising alternative to current treatment against breast cancer.
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