Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1*
...cs of spermatozoa during pre-freezing at 4–5 0C and post-thawing (PT). The data obtained in the form of quantitative data were analyzed descriptively and analysis of variance patterns in One-Way ANOVA. 0 µM, 10 µM, 30 µM and 50 µM genistein treatment in the basic diluent tris aminomethane–egg yolk. Progressive motility post-thawing at 1 hour and 2 hours was highest at genistein concentration 30 µM (P<0.05). The effe...
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
...on of semen was achieved pre-freezing in equilibrated semen and post-thawing in semen thawed in a water bath at 37oC for 30 seconds. Results showed that PEE2 showed the highest (P < 0.05) positive impact on improving progressive motility, livability, abnormality, and acrosome integrity of spermatozoa in pre-freezing and post-thaw semen. All PEE-extenders increased (P < 0.05) viable sperm percentage, and level of total ...
Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*
..., the semen was assessed pre-freezing and post-thaw with parameters such as viability, motility, abnormality, and plasma membrane integrity. The findings revealed that the inclusion of 1% and 2% lycopene in the diluent before freezing exhibited significantly higher (P < 0.05) spermatozoa motility. Moreover, the viability and motility of post-thawed semen significantly improved (P < 0.05) at lycopene doses of 1% and 2%. The addition of 1% to 2% lycopene i...
Sigit Bintara1*, Diah Tri Widayati1 and Pradita Iustitia Sitaresmi2
...lity (76.14±1.35% pre-freezing; 60.00±1.63% after thawing) and viability (85.00±2.00% pre-freezing and 70.14±1.57% after thawing) p<0.05). Regression tests using cubic curves showed that the R square2 values on motilities and viabilities were higher than 0.85, and could be used as a predictor of the higher addition of Curcuma xanthorrhiza. In conclusion, the addition of 3% C. xanthorriza in sem...
Lalu Ahmad Zaenuri*, Enny Yuliani
... factor examined was the pre-freezing duration, which included 10, 15, and 20 minutes (T1, T2, and T3, respectively). The second factor assessed was the post-thawing storage time of 0, 30, 60, 90, and 120 minutes. After equilibration, Eppendorf tubes containing liquid semen were placed four centimeters above liquid nitrogen (LN2) in a Styrofoam container and then frozen by immersion in LN2. Total motility, progressive motility, plasma membrane integrity, and v...
Andi Nirmala1, Abdul Latief Toleng2, Muhammad Yusuf2*, Herdis Herdis3, Athhar Manabi Diansyah2,3, Muhammad Fajar Amrullah2, Rahmat Rahmat2,5, Rajamuddinn Rajamuddin1, Andi Muhammad Alfian1, Hasrin Hasrin4
...3-5°C for 2-3 hours, pre-freezing was done by placing the straw 10 cm above liquid nitrogen for 5-10 minutes, followed by storage in liquid nitrogen at -196°C. For thawing, the straw was immersed in a 37°C water bath for 12 seconds. Semen quality was then evaluated through macroscopic (volume, color, odor, consistency, pH) and microscopic (concentration, motility, viability, membrane integrity, and acrosome integrity) assessments. The data obtained...
