ANTI-TYROSINASE AND ANTI-OXIDANT POTENTIAL OF METHANOLIC EXTRACTS OF SELECTED Citrus bergamia AND Ficus carica PARTS
ANTI-TYROSINASE AND ANTI-OXIDANT POTENTIAL OF METHANOLIC EXTRACTS OF SELECTED Citrus bergamia AND Ficus carica PARTS
Hina Ilyas1, Umar Hanif2, Anser Ali1, Zahid Hassan Tarar3,Hamza Javed4, Tehreem Tahir5, Muhammad Rafiq6*
ABSTRACT
Tyrosinase is a key enzyme in melanogenesis. Its high activity is associated with increased pigmentation causing skin disorders like freckles, melasma and black spots. Therefore, search for new tyrosinase inhibitors is desirable. In present study, methanolic (MeOH) extracts from leaves, fruit peel and pulp of Citrus bergamia (C. bergamia) and, leaves and fruit of Ficus carica (F. carica) were prepared which were further processed for fractional distillation using ethyl acetate (EA), n-hexane (n-Hx) and chloroform (CHCl3) preparing total 20 extracts aiming to test their anti-tyrosinase potential, in-vitro. Our results confirmed that all C. bergamia and F. carica crude extracts showed significant anti-oxidant activity with IC50 range of 384.2 ± 19.1 to 77.3 ± 10.0 μgmL-1, collectively. Moreover, significant anti-tyrosinase activity for C. bergamia (IC50 range = 4.1 ± 0.3 to 366.8 ± 36.5 μg mL-1) and F. carica (IC50 range = 156.5 ± 12.4 to 15.1 ± 2.9 μg mL-1) was found . Interestingly, C. bergamia MeOH-EA peel and C. bergamia MeOH-EA leaves extracts showed IC50 4.1 ± 0.3 and 6.1 ± 1.2 μg mL-1, respectively. Thus, C. bergamia MeOH-EA peel extract with lowest IC50 value among all the tested extracts, is proposed as potent candidate to control tyrosinase rooted hyperpigmentation in future.
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