Antimicrobial properties of Dalbergia, Brassica and Trifolium honey against burn Microorganisms
Antimicrobial properties of Dalbergia, Brassica and Trifolium honey against burn Microorganisms
IRFANA IQBAL1*, PAKEEZA TANWEER1, FARKHANDA MANZOOR1, MUHAMMAD NAUMAN AFTAB2, AFSHAN KALEEM3, ROHEENA ABDULLAH3, ASMA ZAFAR4 & MEHWISH IQTEDAR3
ABSTRACT
This research aimed at assessing the antimicrobial properties of Dalbergia, Brassica and Trifolium honey samples against microorganisms isolated from infected burned skin of patients in children hospital, Lahore, Pakistan. The isolated microorganisms were identified as P. aeruginosa, E.coli, K. pneumoniae and S. aureus. The original bacterial inoculum was serially diluted (adjusted to 1.5 X 106 CFU) and spread on the nutrient agar plates. Whattmann filter paper discs were soaked in three different concentrations (50%, 70% & 90%) of each of the three unifloral honey samples for 48 hrs. The filter discs were placed on the agar plates seeded with the individual bacteria. Solitary effect of antibiotic discs (Ciprofloxacin, Imipenem, Ceftriaxone, Amikacin, and Vancomycin) and their synergistic effect were also studied. No bacterial growth showed resistance to honey at any concentration when used individually or in combination with antibiotic although bacteria showed resistance to Ciprofloxacin and Ceftriaxone. Brassica honey 90% was the most effective at all concentrations with a maximum of inhibition zone 11.13 mm against P. aeruginosa followed by 90% Trifolium honey with the maximum inhibition zone 10.75 mm against P. aeruginosa. The Dalbergia honey (50%) was least effective against P. aeruginosa, however it inhibited S. aureus producing inhibition zone 10.25 mm. Honey-antibiotic combination produced inhibition zone 51 mm that was much larger than the inhibition zone produced by antibiotic or honey when applied individually. Honey, whether used individually or in combination with an antibiotic, was effective against all the bacterial isolates used in this study.
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