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Biological and Molecular Detection of Fig latent trichovirus in Naturally Infected Fig Plants

Biological and Molecular Detection of Fig latent trichovirus in Naturally Infected Fig Plants

Hanaa H.A. Gomaa1*, Dalia Y.Z. Amin1, Mona A. Ismail1 and Khalid A. El-Dougdoug2

1Department of Botany and Microbiology, Faculty of Science, Suez Canal University, Ismailia, Egypt; 2Department of Microbiology, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

 
*Correspondence |Hanaa H.A. Gomaa, Department of Botany and Microbiology, Faculty of Science, Suez Canal University, Ismailia, Egypt; Email: hgoumaa@hotmail.com 

Figure 1:

Field fig trees showing viral symptom on leaves.

Figure 2:

Reaction of host plants inoculated with isolated virus showing none visible symptoms, slightly systemic symptoms on N glutinosa L., N. tabacum cv. white burly, Pepper (Capsucum annum), P. hyprida and tomato, slightly local symptoms on Ch. amaranticolor L. and Ch. quinua.

Figure 3:

Reaction of fig plants grafting inoculated with isolated virus showing latent systemic symptoms none visible symptoms compared with healthy ones.

Figure 4:

The flexible rod viral particles of FLV-1 detected by negative stain TEM.

Figure 5:

Photograph showing the crystal (Cl) and amorphous (Am) inclusion bodies in cells of epidermal (Ep) strips and stomata (St) of infected and healthy N. glutinosa leaves.

Figure 6:

Electrograph of 1.5% agarose gel electrophoresis showing RT-PCR product amplified FLV-CP from extracted RNA from infected fig leaves (1, 2, 3, 4) using (sense CPtr-s and antisense CPtr-s primers . L: DNA ladder weight marker (100 bp ladder).

Figure 7:

The nucleotide sequence of partial CP gene (302 bp) of FLV strain (MZ076515).

Figure 8:

Phylogenetic trees showing the relationship of isolated FLV with Nahavand (KM5167521), I5o5 (FN3775731), Loestanr-1(MG4075531) and Mazandaran, (KM5167521) of fig latent virus isolates recorded in gene bank.

Figure 9:

Translation of partial nucleotide sequence of CP gene for FLV strain (MZ076515) produced 100 amino acids.

Figure 10:

Neighbour joining tree of coat protein gene of FLV-1 CP isolate and 10 isolates published in GenBank based on the amino acid sequence of the CP gene. Numbers represent bootstrap percentage values based on 1000 replicates.

Table 2:

The partial nucleotide sequences producing significant alignments of FLV (MZ076515) CP gene with four FLV isolates recorded in gene bank.

Table 3:

Nucleotide distances and standard error between coat protein gene of FLV isolate and isolates published in GenBank.

Table 4:

Classification and characteristics of Fig latent virus.

Journal of Virological Sciences

July

Vol. 3, Iss. 1

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