Biological and Molecular Detection of Fig latent trichovirus in Naturally Infected Fig Plants
Hanaa H.A. Gomaa1*, Dalia Y.Z. Amin1, Mona A. Ismail1 and Khalid A. El-Dougdoug2
1Department of Botany and Microbiology, Faculty of Science, Suez Canal University, Ismailia, Egypt; 2Department of Microbiology, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
*Correspondence |Hanaa H.A. Gomaa, Department of Botany and Microbiology, Faculty of Science, Suez Canal University, Ismailia, Egypt; Email: hgoumaa@hotmail.com
Figure 1:
Field fig trees showing viral symptom on leaves.
Figure 2:
Reaction of host plants inoculated with isolated virus showing none visible symptoms, slightly systemic symptoms on N glutinosa L., N. tabacum cv. white burly, Pepper (Capsucum annum), P. hyprida and tomato, slightly local symptoms on Ch. amaranticolor L. and Ch. quinua.
Figure 3:
Reaction of fig plants grafting inoculated with isolated virus showing latent systemic symptoms none visible symptoms compared with healthy ones.
Figure 4:
The flexible rod viral particles of FLV-1 detected by negative stain TEM.
Figure 5:
Photograph showing the crystal (Cl) and amorphous (Am) inclusion bodies in cells of epidermal (Ep) strips and stomata (St) of infected and healthy N. glutinosa leaves.
Figure 6:
Electrograph of 1.5% agarose gel electrophoresis showing RT-PCR product amplified FLV-CP from extracted RNA from infected fig leaves (1, 2, 3, 4) using (sense CPtr-s and antisense CPtr-s primers . L: DNA ladder weight marker (100 bp ladder).
Figure 7:
The nucleotide sequence of partial CP gene (302 bp) of FLV strain (MZ076515).
Figure 8:
Phylogenetic trees showing the relationship of isolated FLV with Nahavand (KM5167521), I5o5 (FN3775731), Loestanr-1(MG4075531) and Mazandaran, (KM5167521) of fig latent virus isolates recorded in gene bank.
Figure 9:
Translation of partial nucleotide sequence of CP gene for FLV strain (MZ076515) produced 100 amino acids.
Figure 10:
Neighbour joining tree of coat protein gene of FLV-1 CP isolate and 10 isolates published in GenBank based on the amino acid sequence of the CP gene. Numbers represent bootstrap percentage values based on 1000 replicates.
Table 2:
The partial nucleotide sequences producing significant alignments of FLV (MZ076515) CP gene with four FLV isolates recorded in gene bank.
Table 3:
Nucleotide distances and standard error between coat protein gene of FLV isolate and isolates published in GenBank.
Table 4:
Classification and characteristics of Fig latent virus.