Celecoxib and Cisplatin Synergistically Inhibit Oral Cancer Cell Proliferation Via Modulating the NOTCH 1 Signalling Pathway
Celecoxib and Cisplatin Synergistically Inhibit Oral Cancer Cell Proliferation Via Modulating the NOTCH 1 Signalling Pathway
Zuhair M. Mohammedsaleh1*, Mamdoh S. Moawadh1, Fayez M. Saleh2, Mohammed M. Jalal1, Abdulaziz S Al-Otaibi1, Nizar H. Saeedi1, Rathinasamy Baskaran3, Chih-Yang Huang4 and V. Bharath Kumar5*
Effect of celecoxib and cisplatin on the viability of OC-3 cells. Inhibitory rate of Scc-9 cell viability was determined by the MTT assay when OC-3 cells were treated with; (A) cisplatin, (B) celecoxib (100µM), cisplatin (25 μM) or in combination for 24 h. Data are expressed as the means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group.
Effects of CXB combined with Cis on the migration and invasion of OC-3 cells. Transwell chamber migration and invasion experiments (×100). (A) Transwell chambers images of cell migration; (B) transwell chambers images of cell invasion. (B) EMT proteins- E-cadherin, Vimentin and Claudlin-1 expression was analysed by Western blotting. GAPDH was used as loading control.
Analysis of NOTCH1 pathway in OSCC cells. (A) OC-3 and SCC-9 cells were used to screen for NOTCH1 signaling pathway by western blotting. (B) OC-3 cells were treated with Celecoxib, Cisplatin and combination and further analyzed for NOTCH1, Presenilin1, 2, Nicastrin, Numb, RBPSUH by western blotting. GAPDH and β-actin was used as internal control.
