Expression and Promoter Methylation Status of CDX1 and CDX2 Genes in Chinese Patients with Gastric Cancer
Expression and Promoter Methylation Status of CDX1 and CDX2 Genes in Chinese Patients with Gastric Cancer
Zhihong Huang1, Xuemei Zhang1, Ling Li1, Min Hu1, Mo Chen1 and Qunchao Mei2*
ABSTRACT
Gastric cancer is a multifactorial disease, the fourth most common cancer in the world and the second cause of cancer death. Changes in gene expression levels are one of the most important factors in the occurrence of cancer. Gene promoter methylation changes are considered a suitable target for therapeutic strategies. In the present study, the frequency of this epigenetic phenomenon and the expression of CDX1 and CDX2 genes and their relationship with pathological and clinical characteristics of Chinese patients with gastric cancer were investigated. In this study, in order to investigate the promoter methylation of CDX1 and CDX2 genes, one hundred thirty-three tissue samples were analyzed using the Methylation Specific PCR method and in order to investigate gene expression, sixty-one tumor tissue samples and eleven normal tissue samples were analyzed using the Real-Time RT-PCR method. According to the data obtained, there was no significant difference in the promoter methylation results of CDX1 and CDX2 genes in tumor tissue compared to normal tissues adjacent to the tumor and normal controls. Furthermore, changes in the expression of CDX1 and CDX2 genes show a significant relationship with increasing disease stages and lymph vascular and perineural invasions. It was concluded that increased frequency in the promoter methylation of CDX1 genes in patients with gastric cancer compared to control tissues and its relationship with factors that confirm the poor prognosis of the disease cannot be introduced alone as a possible candidate for further studies to confirm the role of a poor prognostic biomarker in gastric cancer.
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