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Characterization of Aflatoxigenic and Nonaflatoxigenic Isolates of Aspergillus flavus by Cultural and Immunological Methods

Characterization of Aflatoxigenic and Nonaflatoxigenic Isolates of Aspergillus flavus by Cultural and Immunological Methods

Iftikhar Jan* and Sahib Alam

Department of Agricultural Chemistry and Biochemistry, the University of Agriculture, Peshawar, 25130, Pakistan.

 
*Correspondence | Iftikhar Jan, Department of Agricultural Chemistry and Biochemistry, the University of Agriculture, Peshawar, 25130,
Pakistan; Email: ijan719@gmail.com

Figure 1:
Major agroecological zone of north western Pakistan.
Source: ARP-II Diagnostic study 1992.
 
Figure 2:

Formation of orange red color of selected Aspergillus section Flavi isolates on reverse side of AFPA medium plate.

Figure 3:

Total number of Aspergillus section Flavi population isolated from soil and stored maize grains sample taken from different agroecological zones of Khyber Pakhtunkhwa.

Figure 4:

Relative distribution of common dominant fungal genera isolated from field soil and stored maize grains samples taken from different agro-ecological zones of Khyber Pakhtunkwa.

Figure 6:

Room temperature phosphorescence (%) on malt extract agar (MEA) medium modified with β-cyclodextrin (0.3%) and sodium deoxycholate (0.6%) of Aspergillus section Flavi isolated from different agro-ecological zones of Khyber Pakhtunkwa.

Figure 7:

Ammonium hydroxide (NH4OH) vapor induced color change on coconut cream agar (CCA) medium of toxigenic strains (%) of Aspergillus section Flavi isolated from different agro-ecological zones of Khyber Pakhtunkwa.

Figure 8:

Sclerotial production (%) on czapek yeast agar (CYA) medium of aflatoxigenic isolates of Aspergillus section Flavi isolated from different agro-ecological zones of Khyber Pakhtunkwa.

Figure 5:

Blue flourescence (%) on coconut cream agar (CCA) medium of Aspergillus section Flavi isolated from different agro-ecological zones of Khyber Pakhtunkwa.

Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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