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Chemical Pretreatment Method for Enhanced Production of Bioethanol from Sugarcane Bagasse using Bacillus sp.

Chemical Pretreatment Method for Enhanced Production of Bioethanol from Sugarcane Bagasse using Bacillus sp.

Nazish Manzoor1*, Alim Khan1, Muhammad Yaseen1, Abid Ullah1, Zulqarnain2 and Iftikhar Jan2

1Department of Microbiology, Kohat University of Science and Technology, Kohat, Khyber Pakhtunkhwa, Pakistan; 2Directorate of Soil and Plant Nutrition, Agricultural Research Institute, Peshawar, Khyber Pakhtunkhwa, Pakistan.

 
*Correspondence | Nazish Manzoor, Department of Microbiology, Kohat University of Science and Technology, Kohat, Khyber Pakhtunkhwa, Pakistan; Email: nazchemist@qq.com 

ABSTRACT

Sugarcane bagasse (SCB) is usually considered agricultural lignocellulosic biowaste attracting worldwide attention as a potential substitute for fossil fuels derived from the sugar and alcohol industry. In this study, ethanol was produced from the acid and alkaline pretreated samples of sugarcane bagasse using a fermentation process. Amylase was partially purified from the bacterial strain N1 and its activity was checked at distinct pH and temperature. The optimum pH was from 6-8 whereas the temperature was 40 °C. Saccharomyces cerevisiea was added for enhanced fermentation and ethanol production. The acid pretreated sample produced the highest ethanol (15.2%) than the alkaline. The percent production of ethanol and the amount of sugar in pretreated samples was confirmed using High-Performance Liquid Chromatography (HPLC) and sugar contents were increased in acid pretreated samples.

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Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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